【摘要】 用滤纸片法测定黄连素的抑菌活性。结果显示黄连素有较宽的抗菌谱,黄连素对以下五种细菌:革兰氏阴性菌大肠杆菌及革兰氏阳性菌枯草芽孢杆菌、金黄色葡萄球菌的抑制作用较强,对真菌黑曲霉菌的抑制作用稍弱;黄连素抑菌活性不受热处理的影响,而受pH值影响较大,在pH值为4—8范围内,黄连素抑菌活性较好且稳定。采用液体稀释法,研究了黄连素的最小抑菌浓度(MIC)。结果表明,黄连素对金黄色葡萄球菌的最小抑菌浓度为62.5mg/L,对枯草芽孢杆菌的最小抑菌浓度为31.5mg/L,对大肠杆菌的最小抑菌浓度为125mg/L,对黑曲霉的最小抑菌浓度为500mg/L。更多还原

【Abstract】 The antimicrobial effects of Berberine are studied by using disc diffusion method. The results showed that berberine has antibacterial spectrum,inhibitory effect of berberine on bacteria gram negative Escherichia coli and gram positive bacillus subtilis,Staphylococcus aureus,inhibitory effect on disease fungus Aspergillus niger; Berberine antimicrobial activity was not affected by heat treatment,buy by pH instead. In the pH value range of 4- 8,berberine has better antibacterial activity and stability. Minimal inhibitory concentration of berberine( MIC) was determined with the liquid dilution method and the results show that the minimum inhibitory concentration of Staphylococcus aureus was 62. 5 mg /L,the minimum inhibitory concentration of the Bacillus subtilis was 31. 5 mg /L,minimum inhibitory concentrations to the Escherichia coli was 125 mg /L,minimum inhibitory concentration of Aspergillus niger was 500 mg /L.更多还原