【摘要】 目的:观察他克莫司(FK506)对小鼠肠黏膜结构的影响。方法:将制备的小鼠动物模型随机分为实验组和对照组,实验组又分高剂量组(FK506 5 mg/kg灌胃)、中剂量组(FK506 3 mg/kg灌胃)和低剂量组(FK5061 mg/kg灌胃);对照组胃内灌注相同剂量的等渗盐水。每天给FK506,7 d后隔天给药,至30 d收集标本。测量小鼠体重和FK506血药浓度,分别用H-E染色及透射电镜观察小鼠小肠黏膜组织结构和超微结构。结果:实验组小鼠的体重低于对照组,且随FK506剂量加大,体重减轻越明显。实验高剂量组血液中FK506血药浓度最高,中剂量组次之,低剂量组最低。实验组小鼠电镜观察均显示,肠黏膜屏障遭到破坏,且破坏程度与FK506剂量呈正相关。结论:FK506破坏肠黏膜结构是通过破坏肠黏膜细胞间紧密连接等超微结构造成的,且破坏程度与FK506的剂量呈正相关。更多还原

【Abstract】 Objective: To investigate the effects of FK506( Tacrolimus) on the structure of mouce intestinal mucosa. Methods: C57 BL /6 male mice were randomly divided into experimental group and control group. Experimental group included high dose( 5 mg /kg),medium dose( 3 mg /kg)and low dose group( 1 mg /kg). And the mice in control group recieved the same amount saline. In the first week,FK506 was administrated once a day,and after that it was given every other day. Thirty days later,weight of mice and plasma concentration of FK506 were measured. Structure and ultramicro-structure of mouse intestinal mucosa were observed by HE dying and transmission electron microscope( TEM)respectively. Results: The weight of mice in experimental group was lower than that in the control group,and the higher the dose of drug was given,the lighter the body weight become. The plasma concentration of FK506 of high dose group was the highest,medium dose group second and low dose group the lowest. Results of HE dying and transmission electron microscope( TEM) showed that the damage to intestinal mucosal barrier in the experimental group and that the extent of damage was positively correlated with the dosage of drug. Conclusion: FK506 causes damage to the structure of intestinal mucosa.Its mechanism lies in the damage of intestinal mucosa cell tight link and other ultramicro-structures.Moreover,the extent of damage is directly related to the dosage of drug.更多还原