【摘要】 目的了解贵州地区丙型肝炎病毒(HCV)基因型的分布特点及其与感染途径、性别、年龄因素的关系。方法在198份抗HCV抗体和HCV RNA均阳性的血清标本中,分别提取HCV RNA,通过逆转录巢式PCR(RT nested-PCR)扩增C基因的羧基端至E1基因的氨基端长度为474bp的片段,测定其核苷酸序列,与GenBank中已知的HCV序列进行系谱分析,确定HCV基因亚型。结果 198例患者中,HCV 1a型4例(2.0%);1b型例71例(35.9%);2a型9例(4.6%);3a型29例(14.7%),3b型47例(23.7%),6a型37例(18.7%),6d型1例(0.5%)。各基因型在男女性别分布比较,差异无统计学意义(χ2=6.518,P=0.885);不同年龄人群HCV基因型分布比较,差异有统计学意义(χ2=49.056,P=0.005);不同感染途径人群HCV基因型分布比较,差异有统计学意义(χ2=99.245,P=0.005)。结论贵州地区HCV基因型主要为1b型,其次为3b、6a、3a型,同时还存在1a、2a和6d等基因型。HCV基因型与其感染途径有关,基因亚型呈现多样性。更多还原

【Abstract】 Objective To study the distribution of genotypes of hepatitis C virus in Guizhou and its relationship between infectious route of genotype and age,gender was analyzed.Methods Serum specimens in this study were obtained from 198 patients,whose anti-HCV and HCV RNA were positive.A reverse transcriptase PCR(RT nested-PCR)assay using conserved primers deduced from the core-envelope 1(C-E1)region of the hepatitis C virus(HCV)genome was employed to amplify a 474-nucleotide-long fragment.Phylogenetic analysis of the C-E1 sequences was conducted by direct sequencing of the RT-PCR products and alignment with published HCV subtypes in GenBank.Subtypes of the samples were determined by nucleotide sequencing followed by composition of a phylogenetic tree.Results Among the 198 patients surveyed,genotype 1awas detected in 4cases(2.0%),genotype 1bin71cases(35.9%),genotype 2ain 9cases(4.6%),genotype 3ain 29cases(14.7%),genotype 3bin 47cases(23.7%),genotype 6a in 37cases(18.7%)and genotype 6din 1cases(0.5%).Genotype distribution on gender had no statistical significance(P>0.05),and its distribution on people with different ages had statistical significance(P<0.05),and its distribution on patients with different infectious routes was significantly different(P<0.05).Conclusion The major genotypes of HCV are 1b,3b,6aand 3ain Guizhou,and genotype 1ais predominant.Genotypes 1a,2aand 6dexist too.Genotypes of patients infected with HCV are related to their infectious routes,and the HCV genotypes are in a great variety.更多还原