【摘要】 目的通过检测羊水ABH血型物质和序列特异性引物-聚合酶链反应(PCR-SSP)基因技术检测胎儿羊水细胞ABO血型基因型,鉴定胎儿ABO血型。方法选取妊娠16~25周的孕妇53例,抽取羊水,利用间接凝集实验测定羊水ABH血型物质;将羊水细胞进行分离,提取羊水细胞DNA,运用PCR-SSP技术分析其ABO血型基因型。结果 16例羊水标本为非分泌型,占30.2%,37例羊水标本为分泌型,占69.8%;48例羊水标本通过PCR-SSP方法检测出了ABO血型的基因型。经基因鉴定的胎儿羊水细胞ABO血型与羊水分泌型ABH血型物质检测结果一致。结论 PCR-SSP技术可以准确地检测胎儿羊水细胞的ABO血型。更多还原

【Abstract】 Objective To detect amniotic fluid ABH blood group substances and ABO blood group genotype by the polymerase chain reaction with sequence-specific primers(PCR-SSP)to increase the prenatal diagnosis of fetal ABO blood group.Methods 53 pregnant women with gestational age 16-25weeks were selected.Amniotic fluid was extracted for detecting ABH blood group substances by the serological indirect agglutinating reaction;the amniotic fluid cells were separated for extracting DNA.Then the PCR-SSP technique was adopted to analyze the ABO blood group genotypes.Results 16specimens of amniotic fluid were non-secreting type phenotype(30.2%)and 37specimens of amniotic fluid were secreting type phenotype(69.8%);48specimens of amniotic fluid were detected out the ABO blood group genotype by the PCR-SSP method.ABO blood group of fetal amniotic fluid cells by the gene identification was consistent to the detection results of amniotic fluid secreting type ABH blood group substances.Conclusion The PCR-SSP technique can accurately detect the fetal amniotic fluid cells ABO blood group.更多还原