【摘要】 目的探讨乙型肝炎病毒(HBV)慢性化病变患者外周血单个核细胞共刺激分子CD28、CTLA-4及其配体CD80、CD86mRNA的表达及其意义。方法采用Taqman探针实时荧光定量PCR方法分别检测24例慢性乙型肝炎(CHB组)、24例肝硬化(LC组)、28例原发性肝癌(HCC组)和30例健康人(对照组)外周血单个核细胞的共刺激分子CD28、CTLA-4、CD86、CD80mRNA的水平。结果与对照组相比,CD28mRNA表达在CHB组下降显著(t=-2.11,P<0.05);CTLA-4mRNA在疾病各组表达均有不同程度的显著下降:CHB组(t=-2.52,P<0.05),LC组(t=-2.11,P<0.05),HCC组(t=-2.56,P<0.05);CD86mRNA在疾病各组表达也有不同程度的显著下降:CHB组(t=-3.68,P<0.01),LC组(t=-2.99,P<0.01),HCC组(t=-4.42,P<0.01);CD28/CTLA-4mRNA表达在HCC组显著升高(t=2.12,P<0.05);CD80/CD86mRNA表达随着HBV的慢性化进程出现不同程度显著升高:CHB组(t=2.10,P<0.05),LC组(t=2.59,P<0.05),HCC组(t=3.74,P<0.01)。结论 HBV感染患者CD28/B7家族共刺激分子的表达异常与其免疫功能紊乱和慢性化病变发生、发展密切相关。更多还原

【Abstract】 Objective To analyze and evaluate the expression and significance of costimulatory molecules CD28,CTLA-4,CD86, CD80mRNA in peripheral blood mononuclear cells of the patients with HBV chronic change.Methods The levels of costimulatory molecules CD28,CTLA-4,CD86,CD80mRNA in peripheral blood mononuclear cells were detected in 24cases of chronic hepatitis B(CHB),24cases of liver cirrhosis(LC),28cases of hepatocellular cancer(HCC)and 30normal control(NC)subjects by real time quantitative PCR.Results Compared with the NC group,costimulatory molecule CD28mRNA level in the CHB group was significantly decreased(t=-2.11,P<0.05);CTLA-4mRNA level in different diseases groups was decreased to different degrees:the CHB group(t=-2.52,P<0.05),the LC group(t=-2.11,P<0.05)and the HCC group(t=-2.56,P<0.05);CD86mRNA level in different diseases groups was decreased to different degrees too:the CHB group(t=-3.68,P<0.01),the LC group(t=-2.99,P<0.01)and the HCC group(t=-4.42,P<0.01);CD28/CTLA-4mRNA level was significantly increased in the HCC group(t=2.12,P<0.05);CD80/CD86mRNA level was significantly increased to different degrees with the progress of HBV chronic change:the CHB group(t=2.10,P<0.05),the LC group(t=2.59,P<0.05)and the HCC group(t=3.74,P<0.01). Conclusion The expression abnormality of CD28/B7family costimulatory molecules mRNA in HBV infectious patients may be closely related with the immune dysfunction and the development and progression of the chronic change.更多还原