【摘要】 采用两步阳极氧化法在不同的电解液体系和阳极氧化模式下构建钛表面多级孔氧化层。对样品的表面组成、结构和形貌等表面特性进行了表征。通过对MG63细胞在多级孔表面共培养来评价其对成骨细胞粘附、增殖和分化的影响。结果表明:两步阳极氧化法可以在钛表面制备出兼具生物活性和微米/亚微米多级孔结构的晶型TiO2层;与喷砂酸蚀钛表面相比,经两步阳极氧化处理的钛表面更有利于细胞的附着、生长和促进细胞的增殖。更多还原

【Abstract】 The two-step anodization treatment was employed to prepare multi-level surface porous titania layer by the anodic oxidation of pre-treated pure titanium in 2 mol/L CH3 COOH electrolyte with step-galvanostatic mode and then in 1 mol/L H2SO4 electrolyte with potentiostatic mode. The surface morphology and structures were analyzed by scanning electron microscopy and X-ray diffraction, respectively. The effect of the multi-level surface porous structure on the adhesion, proliferation, and differentiation abilities of MG63 cells was evaluated. The results show that the surface-porous bioactive titanium consisting of micro-scale craters overlaid with submicron pores can be prepared by the two-step anodic oxidation. Compared with sandblasted and acid-etched titanium surface, the cell experiments show that the anodized titanium surface with multi-level porous structure can effectively promote osteoblasts adhesion, spreading and proliferation.更多还原