【摘要】 论述了一种新型的磁性聚丙烯酸甲酯微球的制备方法,该方法制备的微球具有良好的磁性和单分散性,并且将其在磁性免疫分析技术中得以运用。首先用化学共沉淀法制备Fe3O4纳米颗粒,PMA种子颗粒由细乳液聚合方法合成,最后用单体溶胀聚合方法合成磁性PMA微球,也就是将PMA种子微球溶解于丙烯酸甲酯磁性流体中。利用氨解反应将氨基偶联在磁性PMA微球表面,进而将HCV抗体通过戊二醛共价偶联到磁性PMA微球表面。微球的单分散性、粒度分布、磁特性分别由扫描电镜、振动试样磁力计测定。实验结果表明:PMA磁性微球的平均尺寸为5.416μm,饱和磁化强度为5.839 Am2/kg,PMA磁性微球上的氨基和HCV抗体偶联量分别由原子吸收光谱仪以及紫外分光光度计测定,测定的氨基浓度为0.659 mmol/g。更多还原

【Abstract】 A novel method for preparation of monodisperse and strong magnetic poly methyl acrylate(PMA) microspheres immobilized with Hepatitis C Virus(HCV) antibody for using in Magnetic Immunoassay was investigated. Fe3O4 nanoparticles were obtained by a chemical co-precipitate method. The PMA seed particles were synthesized by miniemulsion polymerization, and the magnetic PMA microspheres were prepared by monomer swelling polymerization, in which the non-magnetic PMA seed particles were swollen by the methyl acrylate-based magnetic fluid. The amino groups on the surface of magnetic PMA microspheres were obtained by ethylenediamine ammonolysis reaction and then HCV antibody were covalently immobilized on the surface of magnetic PMA microspheres by glutaraldehyde. The morphology, size distribution and magnetic properties of magnetic PMA microspheres were characterized with Scan Electron Microscopy(SEM) and Vibrating Sample Magnetometer(VSM), respectively. The results show that the magnetic PMA microspheres have an average size of 5.416 μm and the saturation magnetization is found to be 5.839(A·m2)/kg. The capacity of amino groups and HCV antibody on the surface of magnetic PMA microspheres were measured by atomic adsorption spectrophotometer(AAS) and UV-Vis spectrophotometer(UV-Vis), respectively. The results reueal that the concentration of the amino groups is 0.659 mmol/g and the HCV antibody has high immobilization efficiency.更多还原