【摘要】 目的探讨各种肝病患者中自身抗体检测在自身免疫性肝病（autoimmune liver disease,ALD）包括自身免疫性肝炎（autoimmune hepatitis,AIH）、原发性胆汁性肝硬化（primary biliary cirrhosis,PBC）及原发性硬化性胆管炎（primary sclerosing cholangitis,PSC）中的临床意义。方法选择我院2013年1月-2014年2月消化科、肝胆外科、移植外科等收治的各种肝病患者225例,分为ALD患者组和其他肝病患者组。用间接免疫荧光法检测225份临床血清标本中抗核抗体（antinuclear antibody,ANA）、抗平滑肌抗体（antismooth muscle antibody,SMA）和抗线粒体抗体（anti—mitochondrial antibody,AMA）,用欧蒙印迹法检测抗肝肾微粒体抗体（liver kidney microsomal,LKM）、抗肝细胞溶质抗原Ⅰ型抗体（liver cytosol—1,LC—1）、抗可溶性肝抗原抗体/抗肝胰抗体（anti—soluble liver antigen antibody/liver pancreas antigen,SLA/LP）、AMAⅡ型（AMA—M₂）并查阅临床资料,对检测结果作出分析。结果 ALD患者组ANA阳性率为77.78%,SMA阳性率为7.41%,AMA阳性率为55.56%,LKM阳性率为7.41%,AMA—M₂阳性率为48.15%,SLA/LP阳性率为7.41%,LC—1阳性率为3.70%。其他肝病患者组ANA阳性率为26.26%,SMA阳性率为4.55%,AMA阳性率为11.11%,LKM阳性率为0.51%,AMA—M₂阳性率为5.05%,SLA/LP阳性率为2.53%,LC-1阳性率为1.52%。ALD患者组自身抗体阳性率与其他肝病患者组相比较,ANA、AMA、LKM、AMA—M₂差异均有统计学意义（P均<0.05）。结论 ALD患者自身抗体检出率较高,为临床医生鉴别病毒性肝炎、ALD及其他肝病提供可靠依据。更多还原

【Abstract】 Objective To investigate the clinical value of autoantibodies detection in autoimmune liver disease（ALD） including autoimmune hepatitis（AIH）,primary biliary cirrhosis（PBC） and primary sclerosing cholangitis（PSC）.Methods From January 2013 to February 2014,225 patients from department of gastroenterology,department of hepatobiliary surgery and transplant surgery were collected.Antinuclear antibody（ANA）,antismooth muscle antibody（SMA） and anti-mitochondrial antibody（AMA） were detected by indirect immunofluorescence method.Liver kidney microsomal antibody（LKM）,liver cytosol-1（LC-1）,soluble antigen/liver pancreatic（SLA/LP） and AMA-M₂ were tested by Europe and Mongolia blotting detection.The clinical datas were consulted and the detected results were analyzed statistically.Results The positive rates of ANA,SMA,AMA,LKM,AMA-M₂,SLA/LP and LC-1 in patients with ALD were 77.78%,7.41%,55.56%,7.41%,48.15%,7.41%and 3.70%,respectively.The positive rates of ANA,SMA,AMA,LKM,AMA-M₂,SLA/LP and LC-1 in patients with other liver diseases were 26.26%,4.55%,11.11%,0.51%,5.05%,2.53%and 1.52%,respectively.There were statistical significance in the differences of positive rates of ANA,AMA,LKM,AMA-M₂ between ALD and other liver diseases group（Pall< 0.05）.Conclusion Autoantibodies of ALD have a higher detection rate,and they provide a reliable basis for clinicians to identify viral hepatitis,ALD and other liver diseases.更多还原