【摘要】 目的探讨血清microRNA的表达差异在卵巢癌诊断和治疗中的临床价值。方法收集149例卵巢肿瘤患者和30例健康体检者,采用qRT-PCR检测受检者血清miR-200a、miR-200b、miR-200c和miR-100的表达情况,对检测结果进行统计学分析。结果 miR-200a、miR-200b和miR-200c在卵巢癌患者血清中表达水平均高于正常对照者,而miR-100在卵巢癌患者血清中表达水平低于对照组,且差异均有统计学意义(P均<0.001)。miR-200a、miR-200b、miR-200c及miR-100在良性肿瘤患者与正常对照者血清中表达水平差异均无统计学意义(P均>0.05)。miR-200a、miR-200b和miR-200c在透明细胞癌的表达水平明显高于浆液性和黏液性癌,且差异均有统计学意义(P均<0.05)。miR-200b在浆液性癌中的表达水平高于黏液性癌,miR-200c在透明细胞癌中的表达水平高于子宫内膜癌,且差异均有统计学意义(P均<0.05)。结论血清miR-200家族的表达可用于卵巢癌的诊断、疗效监测及预后评估。更多还原

【Abstract】 Objective To study the clinical value of microRNA in the diagnosis of ovarian cancer.Methods 149 ovarian tumor patients and 30 healthy people were collected.The expression of serum miR-200 a,miR-200 b,miR-200 c and miR-100 were detected by qRT-PCR,and the results were analyzed statistically.Results The expression of serum miR-200 a,miR-200 b and miR-200 c in ovarian cancer patients were all higher than that of in control group,the expression of the serum miR-100 in ovarian benign tumor patients was lower than that of in control group,and the differences all had statistical significance(Pall< 0.001).There were no statistical significance in the differences of miR-200 a,miR-200 b,miR-200 c and miR-100 levels between ovarian benign tumor patients and control group(Pall> 0.05).The expression levels of serum miR-200 a,miR-200 b and miR-200 c in clear cell carcinoma patients were all higher than that of in both serous and mucinous patients,and the differences all had statistical significance(Pall< 0.05).The expression level of miR-200 b in serous carcinomas patients was higher than that of in mucinous patients,and the expression level of miR-200 c in clear cell carcinoma patients was higher than that of in endometrioid carcinomas,and the differences all had statistical significance(Pall< 0.05).Conclusion The expression of serum miR-200 family can be used for early diagnosis and evaluation of the curative effect and recurrent clinical value in ovarian cancer.更多还原