【摘要】 以四丁基碘化铵为催化剂,采用二甲基一氯硅烷与混悬在有机相中的玻璃酸钠反应,反应滤液经乙酸乙酯沉淀获得硅烷化玻璃酸酯(SHAC)。考察SHAC的皮肤刺激性、细胞毒性及护肤效果,结果表明,家兔背部涂抹质量分数为1%的SHAC溶液,红斑和水肿积分均为0,对皮肤无刺激;SHAC的IC50值大于5 g·L-1,该数值远大于欧盟化妆品无毒性的判定标准1.5 g·L-1,说明其没有细胞毒性;在相对湿度为65%和30%时,使用SHAC的猪皮表面水分含量分别为(49±2.16)%和(45±2.27)%,相对于现在广泛使用的保湿剂玻璃酸钠,SHAC具有良好的保湿效果;在其质量浓度为0.001 g·L-1时,SHAC作用的角质细胞存活率为(106.0±6.2)%,与表皮生长因子(EGF)对比没有显著差异。更多还原

【Abstract】 Silanol hyaluronic acid compound( SHAC) was synthesized from sodium hyaluronate and chlorodimethylsilane suspended in organic phase using tetrabutylammonium iodide as catalyst. The product compound was precipitated by adding ethyl acetate to the filtrate of the reaction product mixture. The skin irritation,cytotoxicity and skin care effect of SHAC product were investigated. In test for back of rabbits that smeared with SHAC of mass fraction 1%,all the cumulative score for erythema and hydropsy is zero. It denotes that SHAC has no skin irritation. IC50 of SHAC is higher than 5 g·L-1that is much higher than 1. 5 g·L-1,which is the EU standard criteria for no cytotoxicity. It shows that SHAC exhibits no cytotoxicity. Under environment of air relative humidy 65% and 30%,the moisture content of pig skin after smeared with SHAC achieves( 49 ± 2. 16) % and( 45 ± 2. 27) % respectively,which is better than that smeared with sodium hyaluronate that is widely used as humectant. The survival rate of keratinocyte that acted with 0. 001 g·L-1mass concentration of SHAC achieves( 106. 0 ± 6. 2) % that is no significant differences as comparing with that acted by epidermal growth factor( EGF).更多还原