【摘要】 目的建立测定复方三黄酊中2组分含量的高效液相色谱法。方法色谱柱:Thermo,ODS-2HYPERSIL,Dim(mm)250×4.6。流动相:甲醇-乙腈-0.025 mol/L磷酸溶液(用三乙胺调节pH为3.0)(15:15:70);流速:1.0ml/min;检测器:DAD检测器;检测波长:280nm和345nm;柱温:25℃。结果黄芩苷在0.06862 ug~0.6862 ug之间进样量与峰面积积分值呈良好的线性关系,平均加样回收率为99.42%,RSD=0.4%(n=6);盐酸小檗碱在0.0198~0.198 ug之间进样量与峰面积积分值呈良好的线性关系,平均加样回收率100.6%,RSD=1.9%(n=6)。结论本法简单易行,可用于复方三黄酊的含量测定。更多还原

【Abstract】 Objective To establish the method for determination of the Baicalin and berberine Hydrochloride in Compound Sanhuang Tincture by HPLC. Methods HPLC chromat ographic condition: Thermo,ODS-2HYPERSIL,Dim( mm) 250 × 4. 6. The mobile phase consisted of methanol-acetonitrile- 0. 025 mol / L phosphoric acid solution( adjusted with triethylamine to pH 3. 0)( 15: 15: 70) at a flow rate of 1. 0 ml·min- 1. The DAD detection wavelength was 280nm and 235nm. The column temperature was 25℃. Results The Baicalin was in good linearity over the range of 0. 06862 ~ 0. 6862 ug. The average recovery was 99. 42%,with RSD of 0. 4%( n = 6). The berberine hydrochloride was in good linearity over the range of 0. 0198 ~ 0. 198 ug. The average recovery was 100. 6%,with RSD of 1. 9%( n = 6). Conclusion This method is accurate,simple and can be used for the determination of Compound Sanhuang Chloramphenicol.更多还原