【摘要】 目的:观察稳心颗粒对缺血性心律失常大鼠心肌组织微小RNA-1(miRNA-1)、缝隙连接蛋白43(Cx43)的影响,以进一步探讨稳心颗粒抗心律失常的作用机制。方法:30只SD大鼠,随机分为假手术组、模型组和治疗组。结扎大鼠左冠状动脉前降支制作缺血性心律失常模型,稳心颗粒治疗组在术前7d灌胃给药。假手术组大鼠仅开胸,不施行冠脉结扎。取大鼠心肌组织,采用实时荧光定量聚合酶链反应、免疫组织化学法及Western Blotting等方法,检测大鼠心肌组织中miRNA-1及Cx43蛋白表达。结果:模型组左室心肌组织miRNA-1表达水平明显升高(P<0.01),为假手术组的(4.67±1.21)倍,治疗组miRNA-1表达水平明显降低(P<0.01),仅为模型组的(0.39±0.12)倍。模型组左室心肌组织Cx43蛋白相对含量显著低于假手术组(P<0.01);稳心颗粒治疗后,Cx43蛋白相对含量明显升高(P<0.01)。结论:稳心颗粒可能通过抑制miRNA-1的表达,减轻对Cx43的转录后抑制,使表达分布异常的Cx43得以恢复,最终发挥防治缺血性心律失常的作用。更多还原

【Abstract】 Objective: To observe the effect of Wenxin Granules on the expression of microRNA-1 and Connexin43(Cx43) in the myocardial tissue of ischemic arrhythmia rats and further explore the mechanism of anti-arrhythmic effects of Wenxin Granules. Methods: Thirty SD rats were randomly divided into sham-operated group, model group and treatment group. The ischemic arrhythmia rats models were induced by ligation of the left anterior descending coronary artery. Wenxin Granules was given 7 days before surgery, the rats in sham-operated group only given thoracotomy but without coronary ligation. After taking myocardial tissue from rats, the expression of miRNA-1 and Cx43 were detected by real-time quantitative PCR, immunohistochemistry and Western Blotting. Results: The expression level of miRNA-1 in model group increased signii cantly(P<0.01) and(4.67±1.21) times than sham-operated group, the expression level of miRNA-1 decreased in treatment group(P<0.01) and(0.39±0.12) times of model group. The relative content of Cx43 on myocardial tissue in model group signii cantly lower than sham-operated group(P<0.01), after treatment with Wenxin Granules, the relative content of Cx43 increased obviously(P<0.01). Conclusion: Wenxin Granules may reduce post-transcriptional suppression of Cx43, recover normal expression distribution of Cx43 by inhibiting the expression of miRNA-1, eventually played a role in the prevention and treatment of ischemic arrhythmia.更多还原