【摘要】 目的:探讨再生障碍性贫血(AA)患者外周血和骨髓中白细胞介素17(IL-17)、白细胞介素23(IL-23)及转化生长因子β(TGF-β)的表达水平及其临床意义,阐明AA的发病机制。方法:随机选取50例AA患者作为实验组,30例同期体检健康人作为对照组;采用酶联免疫吸附法(ELISA)和实时荧光定量PCR法检测外周血、骨髓中IL-17、IL-23、TGF-β蛋白及mRNA表达水平。结果:与对照组比较,实验组患者外周血、骨髓中IL-17和IL-23蛋白及mRNA表达水平明显升高,组间比较差异有统计学意义(P<0.01),其中以IL-17升高最为显著(P<0.001);实验组患者外周血和骨髓中TGF-β蛋白及mRNA表达水平明显降低,组间比较差异亦有统计学意义(P<0.01)。实验组患者外周血和骨髓中IL-17与IL-23蛋白表达水平均呈正相关关系(r=0.69,P<0.05;r=0.71,P<0.05),IL-17与TGF-β蛋白表达水平呈负相关关系(r=-0.65,P<0.05),IL-23与TGF-β蛋白表达水平呈负相关关系(r=-0.72,P<0.05)。结论:IL-17、IL-23和TGF-β可能直接参与AA的发生发展过程,三者的联合检测可为AA患者的临床治疗提供新的思路。更多还原

【Abstract】 Objective To explore the expression levels of IL-17,IL-23and TGF-βin peripheral blood and bone marrow of the patients with aplastic anemia(AA)and its clinical significances,and to clarify the pathogenesis of AA.Methods 50cases of AA patients were randomly selected as experiment group and 30cases of healthy people at the same period were used as control group;Enzyme-linked immunosorbent assay(ELISA)and real-timefluorescence quantitative were performed to detect the expression levels of IL-17,IL-23and TGF-βproteins and mRNA in peripheral blood and bone marrow.Results Compared with control group,the protein and mRNA levels of IL-17and IL-23in peripheral blood and bone marrow of the patients in experiment group were significantly increased,and the differences between two groups were statistically significant(P<0.01),among them,the elevated IL-17was significant(P<0.001);the protein and mRNA levels of TGF-βin peripheral blood and bone marrow of the patients in experiment group were significantly decreased,and the differences between two groups were also statistically significant(P<0.01).The protein and mRNA levels of IL-17and IL-23in peripheral blood and bone marrow of the patients in experiment group had positive correlation(r=0.69,P=0.041;r=0.71,P< 0.05),while the protein levels of IL-17and TGF-βhad negative correlation(r=-0.65,P<0.05);and the potein levels of IL-23and TGF-βhad negative correlation(r=-0.72,P<0.05).Conclusion IL-17,IL-23and TGF-βmay participate in the occurrence and development of AA,and the combined detection of the three indexes provides the new ideas for clinical treatment of AA.更多还原