【摘要】 目的了解河北汉族人群Duffy血型系统抗原分布状况,建立河北汉族人群稀有血型抗原初步资料,解决抗-Fya等稀有血型抗体患者的用血问题。方法随机抽取无血缘关系的河北汉族健康人群血样5mL(EDTA抗凝),采用抗球蛋白检测卡法,按照说明书进行献血者Fya、Fyb抗原检测,对于检测出的Fya阴性标本,采用SSP法进一步确认抗原基因状况。结果在1 901份献血者标本中Fya+b+233例(12.26%),Fya+b-1 663例(87.48%),Fya-b+5例(0.26%),未检测出Fya-b-标本。Fya基因频率为0.936 1,Fyb基因频率为0.063 9。经Hardy-Weinberg平衡检验,观察值与预期值之间差异无统计学意义(χ2=1.123 2,P>0.05),河北汉族人群中Duffy血型系统抗原分布符合Hardy-Weinberg平衡。结论在河北汉族人群中Fya阴性表型分布很低,一旦患者产生抗-Fya,通过随机筛查试验找到相合的供者非常困难,严重时会危及到患者的生命,因此,实验室有必要建立Fya阴性献血者资料库,以便及时为患者提供合适的血液成分。更多还原

【Abstract】 Objective To investigate the distribution of Duffy blood type antigen in the Han nationality in Hebei and to accumulate the first-hand data of rare blood type antigen in Hebei Han population,in order to solve the blood type incompatibility of patients who have rare blood type antibody such as anti-Fya. Methods Blood samples from unrelated blood donors were tested to detect the Fya,Fybantigen using the antiglobulin test card method according to the instruction. If Fyanegative specimens were detected,the SSP method was adopted for further confirmation of the antigen gene. Results In 1 901 specimens from blood donors 233 cases of Fya + b +( 12. 26%),1 663 cases of Fya + b-( 87. 48%),5 cases of Fya- b +( 0. 26%) were detected,and Fya- b-was undetected. The gene frequency of Duffy blood types were Fya= 0. 9361,Fyb= 0. 0639. And that was in accordance with Hardy Weinberg equilibrium( χ2= 1. 1232,P > 1. 1232). Conclusion The frequency of Fya-phenotypic in Hebei Han nationality is low and our results suggest that it is very necessary to establish a Fyanegative blood donor database,since that random screening test is very difficult to find consistent donor for patients with anti-Fyaantibody.更多还原