【摘要】 减量训练对于防止运动性疾病和过度疲劳的发生起重要作用,对于促进心脏朝着有利于运动员机能提高的方向发展至关重要。研究利用72只SPF级7周龄雄性SD大鼠,经过1周适应性训练后,随机分为8组:3周大运动量训练组、3 d、6 d和2周减量训练组及其相应的对照组。3周大运动量训练采用中等强度和大强度交替进行,运动时间逐渐延长,然后进行减量训练。训练后取左心室肌组织,测定PI3K、Akt的蛋白和磷酸化采用Western blotting方法。结果显示:减量训练过程中,心肌PI3K P85蛋白表达有升高趋势。PI3K是减量训练诱导心肌保护的重要调节物。不同的运动负荷对Akt蛋白表达量和磷酸化产生不同的影响。在3周大运动量训练后进行减量训练,心肌Akt蛋白表达显著增加。在3周大运动量训练后进行6 d减量训练,AktSer473磷酸化显著增加,2周减量训练后,心肌AktSer473磷酸化显著增加。减量训练过程中,心肌Akt蛋白表达和AktSer473磷酸化有升高趋势。减量训练通过增加Akt蛋白表达和AktSer473磷酸化对心肌起保护作用。更多还原

【Abstract】 Tapering is important to prevent exercise diseases and overtraining,also it’s benefit for heart to improve exercise performance. Seventy-two 7 weeks old male SD rats were in this study. After one week adaptability training they were divided into 8 groups,including 3 weeks high volume training group,3 days tapering group,6 days tapering group,2 weeks tapering group,and all the paralleled control groups. The rats in 3 weeks high volume training group took moderate intensity exercise and high intensity training alternatively,the training time were gradually increased,then rats took tapering. Tapering was including 3 days,6 days and 2 weeks respectively for different groups. After training,the left ventricle cardiac muscle was collected,and the protein expressions and protein phosphorylation of PI3K and Akt were detected by Western blotting. The results showed that PI3K P85 protein expression in cardiac muscle had increased during tapering. It indicated that PI3K was an important modulation for cardiac muscle. Different loads training had different effects on Akt protein expression and Akt phosphorylation. The expression of protein Akt in cardiac muscle increased significantly after 3 weeks high volume exercise combined tapering. The phosphorylation of protein AktSer473increased significantly after 3 weeks high volume training combined 6 days tapering,as well as after 2 weeks tapering. The expression of protein Akt and phosphorylation of protein AktSer473in cardiac muscle had increasing trends during tapering. Results demonstrated that tapering could protect cardiac muscle by increasing Akt protein expression and AktSer473phosphorylation.更多还原