【摘要】 以‘中梨一号’的单芽茎段为外植体,建立‘中梨一号’离体培养体系。试验表明,‘中梨一号’取材的最佳时间为5月中旬;基本培养基、6-BA和NAA这3种因素对‘中梨一号’芽增殖影响差异较大,按影响大小依次为6-BA、基本培养基、NAA;最佳的‘中梨一号’芽增殖的培养基为1/2MS+6-BA1.0mg/L+NAA 0.2 mg/L+GA30.5 mg/L,40 d后增殖系数达4.6;适宜‘中梨一号’组培苗的生根培养基为1/2MS+IBA1.0 mg/L,生根率达44.8%。更多还原

【Abstract】 Single bud stem of ‘Zhongli1’ pear were used as explants to establish in vitro culture and rapid propagation system. Results showed that mid-May was the best sampling time for ‘Zhongli1’ pear, effect of culture medium, 6-BA and NAA on proliferation coefficient has great difference, that factors followed a descending order of 6-BA, culture medium, NAA; the 1/2MS medium with 1.0mg/L6-BA+0.2mg/LNAA was optimal for the shoots multiplication, the proliferation coefficient was up to 4.6 after culturing for 40 days;the 1/2MS medium with 1.0mg/L IBA was optimal for rooting in vitro, rooting rate could be up to 44.8%.更多还原