This study aimed to construct recombinant adenovirus expressing Epstein-Barr nuclear antigen 1(EBNA1)against nasopharyngeal carcinoma(NPC).The C-terminal region fragment of the ebna1gene of Epstein-Barr virus was amplified from the standard strain B95-8by polymerase chain reaction(PCR).The gene fragment was inserted into the pDC316shuttle plasmid using the EcoRI and BglII restriction enzyme sites.The pDC316-ebna1shuttle plasmid and pBHG helper plasmid were cotransfected into HEK293cells after sequencing.The...