【摘要】 目的:探讨荧光定量PCR(FQ-PCR)检测乙型肝炎病毒(HBV)-DNA的临床意义。方法:采用FQ-PCR检测265例乙型肝炎(乙肝)患者和150名健康体检者的血清HBV-DNA,并将结果与酶联免疫吸附试验测定的乙肝血清标志物进行比较。结果:不同血清标志物模式的各组均可检出HBV-DNA阳性,其中HBsAg、HBeAg、HBcAb阳性组和HBsAg、HBeAg阳性组的HBV-DNA阳性率和病毒拷贝数均显著高于其他血清标志物模式组和对照组(P<0.01)。HBV所致不同疾病类型的HBVDNA阳性率差异均无统计学意义(P>0.05),但急性乙肝病毒拷贝数均明显高于其他疾病(P<0.01)。结论:FQ-PCR检测HBV-DNA可以直接反映是否存在HBV感染,判断病毒的复制能力和传染性,指导临床用药和观察疗效,具有重要的临床意义。更多还原

【Abstract】 Objective: To investigate the clinical value of fluorescent quatititive polymerase chain reaction( FQ-PCR) in the detection of hepatitis B Virus( HBV)-DNA. Methods: The positive rate and copies of HBV-DNA in 265 HBV patients and 150 healthy people were detected using FQ-PCR,which were compared with the serological markers of hepatitis B detected by enzyme linked immunosorbent assay. Results: The HBV-DNA was detected in all cases with different serological markers,the positive rate and copies of HBV-DNA in HBsAg,HBeAg and HBcAb( +) group and HBsAg and HBeAg( +) group were higher than those in other groups( P <0.01). The difference of the positive rate of HBV-DNA in different diseases types caused by HBV was not statistically significant( P >0.05),the copies of HBV in acute hepatitis patients were significantly higher than that in other groups( P <0.01). Conclusions:The HBV-DNA detected by FQ-PCR can directly reflect the HBV infection,judge the ability of replication and infection,guide the clinical medication and observe curative effects.更多还原