【摘要】 目的:研究3-溴丙酮酸(3-BrPA)联合阿霉素(ADM)对乳腺癌细胞MDA-MB-231增殖及凋亡的影响。方法:采用3-BrPA 80、160、320μmol/L作用于MDA-MB-231乳腺癌细胞18 h后,测定其对细胞内ATP的影响;分别用3-BrPA 10、20、40、80、160μmol/L和ADM 0.75、1.5、3、6、12μmol/L,以及3-BrPA 80μmol/L与ADM 0.75、1.5、3、6、12μmol/L合用作用于MDA-MB-231乳腺癌细胞24、48和72 h后,MTT法检测乳腺癌细胞MDA-MB-231增殖情况;3-BrPA 80μmol/L组、ADM0.75μmol/L组以及3-BrPA 80μmol/L与ADM 0.75μmol/L合用组作用于乳腺癌细胞MDA-Mb-231 24 h后,利用碘化丙啶染色,流式细胞仪检测其诱导乳腺癌细胞凋亡的影响;采用3-BrPA 16μmol/L、ADM 0.2μmol/L以及3-BrPA 16μmol/L与ADM0.2μmol/L合用作用于乳腺癌细胞MDA-MB-231,5 d后观察对集落克隆形成的影响。结果:3-BrPA对乳腺癌细胞MDA-MB-231 ATP的生成有抑制作用;3-BrPA联合ADM后可明显增强ADM的细胞毒性作用;3-BrPA 80μmol/L与ADM 0.75μmol/L合用组诱导乳腺癌细胞MDA-MB-231 24 h凋亡率为39.6%,均显著高于对照组、3-BrPA单用组和ADM单用组(P<0.01);3-BrPA增强ADM对乳腺癌细胞MDA-MB-231集落克隆形成的抑制作用。结论:3-BrPA可以增强ADM对乳腺癌细胞MDAMB-231增殖的抑制作用以及增强ADM诱导乳腺癌细胞凋亡的作用。更多还原

【Abstract】 Objective: To investigate the effect of 3-Bromopyruvate( 3-BrPA) combined with adriamycin( ADM) on proliferation and apoptosis of human breast carcinoma cells in vitro. Methods: The effects of 3-BrPA at 80,160 and 320 μmol / L on ATP production of MDA-MB-231 breast cancer cells were detected. The growth inhibition effect was detected by MTT assay which induced by 3-BrPA( 10, 20,40,80,160 μmol / L),adriamycin( 0. 75,1. 5,3,6,12 μmol / L) and 3-BrPA at 80 μmol / L combined with adriamycin( 0. 75,1. 5, 3,6,12 μmol / L) for 24 h,48 h and 72 h in MDA-MB-231 breast cancer cells. Cells were treated with 3-BrPA( 80 μmol / L), adriamycin( 0. 75 μmol / L) and 3-BrPA( 80 μmol / L) combined with adriamycin( 0. 75 μmol / L) for 24 hours,the apoptotic cells were detected using propidium iodide staining by flow cytometry. Cells were treated with 3-BrPA( 16 μmol / L),adriamycin( 0. 2 μmol / L) and 3-BrPA( 16 μmol / L) combined with adriamycin( 0. 5 μmol / L) for 5 days,the colony formation was detected by crystal violet staining method. Results: 3-BrPA inhibited the formation of ATP in MDA-MB-231 breast cancer cells; 3-BrPA combined with ADM significantly enhanced the cytotoxicity of ADM. When MDA-MB-231 breast cancer cells were treated with 3-BrPA at 80 μmol / L combined with ADM at 0. 75 μmol / L,the apoptosis rate was 39. 6%,which was significantly higher than that in control group,3-BrPA group and ADM group( P < 0. 01). 3-BrPA enhanced the inhibitory effect of ADM on colony formation of MDA-MB-231 breast cancer cells. Conclusions: 3-BrPA can enhance the inhibitory effect of adriamycin on proliferation and apoptosis of MDA-MB-231 breast cancer cells.更多还原