【摘要】 目的通过主动全身过敏试验(ASA)及被动皮肤过敏试验(PCA)对几种临床易于发生Ⅰ型过敏反应的中药注射剂的致敏性进行再评价。方法取生理盐水、卵蛋白、清开灵注射液、生脉注射液、丹参注射液、喜炎平注射液对豚鼠进行主动全身过敏试验和大鼠被动皮肤过敏试验:(1)Elisa法检测主动全身过敏试验中采集的豚鼠血浆中IgE、组胺、类胰蛋白酶、β-氨基己糖苷酶的含量。(2)观察被动皮肤过敏试验中大鼠背部出现的蓝斑情况。结果主动全身过敏试验中卵蛋白组、生脉注射液组、丹参注射液组豚鼠血浆中IgE、组胺及β-氨基己糖苷酶含量均高于生理盐水组;被动皮肤过敏试验中卵蛋白组、生脉注射液组、丹参注射液组可见明显蓝斑。结论主动全身过敏试验结合被动皮肤过敏试验表明卵蛋白、生脉注射液、丹参注射液、清开灵注射液有致敏性,可引起Ⅰ型过敏反应的发生。更多还原

【Abstract】 Objective To reevaluate the allergenicity of several Chinese medicine injection which can cause TypeⅠhypersensitivity in clinical pracitce using the active systemic anaphylaxis( ASA) and passive cutaneous anaphylaxis test( PCA). Methods The guinea pig active systemic anaphylaxis test and the rat-homologous passive cutaneous anaphylaxis test were carried out on the normal saline group,ovalbumin group,Qingkailing injection,Shengmai injection,Danshen injection group and Xiyanping injection group. The contents of IgE,histamine,tryptase and β-hexosaminidase in plasma of guinea pigs were determined by ELISA assay. Whether there was a blue spot in the back of PCA rats was observed. Results In the ASA test,the contents of IgE,histamine and β-hexosaminidase in plasma of guinea pigs of ovalbumin group,Shengmai injection group and Danshen injection group were higher than that in the normal saline group.In the passive cutaneous anaphylaxis test,ovalbumin group,Shengmai injection group and Danshen injection group showed obvious blue spots. Conclusions Ovalbumin,Shengmai injection,Danshen injection and Qingkailing injection can cause the occurrence of type Ⅰhypersensitivity reaction.更多还原