[Objective] The research aimed to understand the distribution situations of brucellosis in blue sheep in some regions of Helan Mountains in Yinchuan of Ningxia. [Method]53 DNA samples extracted from blue sheep were taken as template to make PCR amplification with P1 and P2 primers. The distribution situations of brucellosis in blue sheep in Helan Mountains were detected. [Result] The positive rate of brucellosis in 53 samples of blue sheep was zero. [Conclusion] The research results showed that no brucellos...