[Objective] In order to improve resistant of soybean to lepidopterons insects,Cry1 C gene was transformed into soybean,in which Cry1 C is one of the most important insect-resistant gene to the Lepidoptera. [Method]This study transformed insect-resistant genes Cry1 C into soybean varieties Williams82 by Agrobacterium-mediated,and then,Bar test papers and PCR were used to identify transformed plants. [Result] Finally,28 positive transformation of soybean plants were gained. [Conclusion] Initially identified t...