【摘要】 [目的]为研究饥饿胁迫下鱼类的生理生化变化提供参考。[方法]采用传统瑞氏染色法研究饥饿胁迫下大鳞副泥鳅外周红细胞的形态变化。[结果]大鳞副泥鳅的成熟红细胞逐渐发生变化,先出现核膜溶解,然后出现细胞形变,接着出现核质扩散和细胞膜溶解,最后整个成熟红细胞膜完全溶解,核染色反应由紫黑色变为紫红色最后变为粉红色。大鳞副泥鳅的幼稚红细胞则明显增多。[结论]在饥饿胁迫下大鳞副泥鳅外周红细胞出现核异常,主要表现为核膜溶解和核质扩散,因此红细胞核异常可以作为饥饿胁迫的指标。更多还原

【Abstract】 [Objective]The research aimed to provide references for studying the physiological and biochemical changes of fishes under starvation stress.[Method]Using traditional Wright’s staining method,the morphology changes of peripheral erythrocyte in Paramisgurnus dabryanus under starvation stress were studied.[Result]The morphology of mature erythrocyte in P.dabryanus gradually changed.First,the nuclear membrane was dissolved,then the cell deformation occurred and the nucleoplasm diffused and cytomembrane began to dissolve,finally the membrane of mature erythrocyte completely dissolved.The staining reaction of nuclear was from purple-black to purple-red,and finally turned to pink.The The number of immature erythrocyte in P.dabryanus was significantly increased.[Conclusion]The nuclear anomalies of peripheral erythrocyte in P.dabryanus under starvation stress occurred,and their manifestations were the dissolution of karyolemma and diffusion of karyoplasms.Therefore the nuclear anomalies of erythrocyte could be used as the index of starvation stress.更多还原