【摘要】 [目的]建立快速、有效检测农产品中玉米赤霉醇(ZER)残留量的方法。[方法]试验以人工合成的ZER为包被原,ZER为竞争半抗原,两者与一定量的抗玉米赤霉醇单克隆抗体反应,应用间接竞争ELISA法建立玉米赤霉醇单克隆抗体快速检测农产品中ZER含量的半定量检测,同时对该ELISA试剂盒进行了调试。[结果]试验建立检测ZER的ELISA方法,其最适包被浓度为1∶5 000,抗体最适工作浓度为1∶2 500,酶标二抗的最适工作浓度为1∶5 000。试剂盒检测限为0.5 ng/ml,添加回收率在70.0%~116.0%,变异系数小于20%,在2~8℃条件下可保存90 d。[结论]该试验建立的方法可应用于玉米、小麦、高粱等中的玉米赤霉醇的检测,应用前景广阔。更多还原

【Abstract】 [Objective]To establish a method for rapidly and effectively detecting ZER in agricultural products. [Method]With ZER package as original,ZER as hapten for competition,both with a certain amount of resistance to zeranol monoclonal antibody response. The indirect competitive ELISA method was used to establish the zeranol monoclonal antibody rapid detection ZER content in the semi-quantitative detection of agricultural products. At the same time the ELISA kit was tested. [Result]ELASA method was established to detect ZER,the optimal concentration of package,IgG-HRP are 1∶ 5 000,the optimal concentration of antibody is 1∶ 2 500. The detection limit of kit is 0. 5 ng /ml,adding recovery rate 70. 0%- 116. 0%,variation coefficient is less than 20%,which can be stored for 3 months under 2- 8 ℃. [Conclusion]The method can be used in detection of ZER in maize,wheat,sorghum with broad prospect.更多还原