【摘要】 [目的]对采自内蒙古根河地区的灰紫香蘑进行组织分离与鉴定。[方法]分别选取菌盖处、菌褶与菌柄交界处和菌柄处进行组织分离,将分离物进行ITS序列分析,计算遗传距离,并采用邻接法构建NJ系统发育树。[结果]菌褶与菌柄交界处为最佳分离部位,菌丝生长速度快、洁白细密、污染率低;其次,子实体自然风干3 d后再进行分离可有效降低菌丝污染程度;最后分离物经ITS序列测定,系统发育分析证实其为灰紫香蘑。[结论]该方法获得了灰紫香蘑的纯培养菌株,为进一步开发和利用灰紫香蘑提供了科学依据。更多还原

【Abstract】 [Objective]To isolate,cultivate and accurately identify Lepista glaucocana which was collected from Genhe district in Inner Mongolia. [Method]Different tissues from pileus,stipe,junction of pileus and stipe were isolated,then the isolate was identified by internal transcribed spacer( ITS),the genetic distance was calculated,and a NJ systematic tree was established by the neighbor-joining method. [Result]The results showed that junction of pileus and stipe is the best isolation position,which mycelia with fast growth and low pollution; Secondly,it is lower pollution when airdry the fruiting body for 3 days; At last,the isolate was identified as L. glaucocana analyzed by phylogenetic relationship of ITS gene sequences. [Conclusion]The pure strain was obtained which can provide a scientific basis for development and use of L. glaucocana.更多还原