【摘要】 [目的]研究碳源和氮源对人工栽培花脸香蘑(Lepista sordida)菌丝生长和多糖产量的影响。[方法]以人工栽培花脸香蘑菌株为研究对象,利用液体培养的方法,通过比较分析菌丝体麦角固醇含量和多糖产量,研究不同碳源和氮源的影响。[结果]通过比较分析麦角固醇含量结果显示,果糖、甘露糖、纤维二糖、蔗糖、葡萄糖和麦芽糖都可以作为花脸香蘑菌丝体生长的碳源,但相比之下,前三者生长效果更好,选择果糖作为碳源,多糖产量最高;丙氨酸、甘氨酸、黄豆粉和蛋白胨都可以作为氮源使用,其中以丙氨酸为氮源生长效果最好,以黄豆粉为氮源的多糖产量最高。[结论]该研究可为花脸香蘑菌丝体的研究与开发利用提供理论依据。更多还原

【Abstract】 [Objective]The aim is to study the effect of carbon and nitrogen sources on the growth and polysaccharide outputs of artificial Lepista sordida mycelia. [Method]The strain of artificial L. sordida was investigated for carbon and nitrogen nutrition by ergosterol contents and the production of polysaccharide in liquid media. [Result]The result shows that fructose,mannose,cellobiose,sucrose,glucose and maltose can be used as a carbon source for L. sordida strain by analyzing ergosterol contents. And the former three carbon materials are better for mycelia growth in contrast with sucrose,glucose and maltose. In the meantime,we can get the highest polysaccharide yield with fructose as a carbon source. Alanine,glycine,soybean powder and peptone can be used as a nitrogen source. Alanine is best for mycelia growth and bean powder can produce the highest polysaccharide. [Conclusion]The result provides theoretical basis for the study and production of L. sordida mycelia.更多还原