【摘要】 Δ8去饱和酶和Δ9链延长酶是二高-γ-亚麻酸(DGLA)和二十碳四烯酸(ETA)合成的关键酶。本研究利用大豆种子特异性启动子替换35S启动子构建了新的多基因辅助载体PAUX-2,并在此基础上构建了包含来自小眼虫藻的Δ8去饱和酶基因和来自球等鞭金藻的Δ9链延长酶基因的植物表达载体pCambia2300-Δ8Δ9。为进一步利用转基因技术研究这些基因在植物中的表达提供了条件。更多还原

【Abstract】 The Δ8 desaturase(Δ8Des) and Δ9 elongase(Δ9Elo) are the key enzymes for the synthesis of dihomo-γ-linolenic acid(DGLA) and eicosatetraenoic acid(ETA).In the research,a new assistant expression vector,PAUX-2,was constructed through replacing the 35S promoter with a soybean seed-specific promoter BCSP952.Then a plant expression vector,pCambia2300-Δ8Δ9,simultaneously containing Δ8Des gene from Euglena gracilis and Δ9Elo gene from Isochrysis galbana,was constructed,which provided essential tool to study the expression of these exogenous genes in plants.更多还原