【摘要】 以酵母EGY48为材料,研究了不同培养时间(12、16、20、24、30 h)、细胞状态(OD600)、电击电压(600、800、1 000、1 200、1 400 V)及不同质粒DNA用量(1、2、5、10、15、20μg)对酵母电击转化效率的影响。结果表明:当培养时间为20 h、OD600=1.4、电击电压为1 000 V、质粒用量为5μg时,该系统转化效率最高,为后续高效文库的筛选奠定了基础。更多还原

【Abstract】 Using S. cerevisiae EGY48 as material,the effects of different incubation times( 12,16,20,24 and 30 h),cell conditions( OD600),electric voltages( 600,800,1 000,1 200 and 1 400 V) and plasmid DNA dosages( 1,2,5,10,15 and 20 μg) on transformation efficiency were studied. The results showed that when the incubation time was 20 h,OD600value was 1. 4,electric voltage was 1 000 V and plasmid dosage was 5 μg,the transformation efficiency was the highest,which laid a foundation for further screening of efficient library.更多还原