【摘要】 目的:探讨冠心II号方对心肌缺血再灌注样损伤的保护作用。方法:体外培养心肌细胞,造成缺氧/复氧模型,模拟心肌缺血再灌注样损伤,实验分为含10%空白血清的空白对照组、10%冠心II号血清的实验组和10%合心爽血清的阳性对照组,检测心肌细胞培养上清液中乳酸脱氢酶(LDH)活性、磷酸肌酸激酶(CK)活性、丙二醛(MDA)含量及心肌细胞中超氧化物歧化酶(SOD)活性变化。结果:与空白对照组相比,冠心II号组和阳性对照组心肌细胞培养上清液中LDH活性明显降低(P<0.05);CK的活性也显著下降(P<0.01),MDA含量明显减少(P<0.05);而心肌细胞中SOD活性显著增强(P<0.01)。结论:中药复方冠心II号对心肌细胞缺血再灌注样损伤具有明显的保护作用。更多还原

【Abstract】 OBJECTIVE: To investigate the protective effects of Guanxin II Hao in myocardial ischemia- reperfusion injury. METHODS:Myocardial cells were cultured in vitro in a hypoxia/reoxygenation model,simulating myocardial ischemia reperfusion injury. The experiment was divided into 10% blank serum control group,10% Guanxin II Hao serum experimental group and 10% gathers the heart crisp serum the masculine control group. Myocardial cells with lactic acid dehydrogenase(LDH),creatine kinase(CK) release and malondialdehyde(MDA) content and superoxide dismutase(SOD) activity was evaluated. RESULTS:Guanxin II Hao group compared with the blank serum group, supernatant of cultured LDH release was significantly reduced(P<0.05); the amount of released CK was also decreased significantly(P<0.01). MDA content decreased significantly(P<0.05); whilst SOD activity was significantly enhanced(P<0.01). CONCLUSION:This Chinese medicine compound used for treating coronary heart disease showed protective effects on myocardial hypoxia / reoxygenation injury in vitro.更多还原