【摘要】 目的探讨肝复健方抗肝纤维化(HF)的可能作用机制。方法将60只SD大鼠随机分为正常组、模型组、肝复健方低剂量组、肝复健方中剂量组、肝复健方高剂量组、秋水仙碱组各10只,除正常组外其余各组腹腔注射猪血清0.5ml/只制备HF模型,每周2次,共8周。造模成功后,肝复健方低、中、高剂量组分别给予含1.4g/ml、2.8g/ml、4.2g/ml肝复健方水煎剂灌胃;秋水仙碱组给予秋水仙碱片混悬液0.154mg/kg;正常组和模型组给予10ml/kg生理盐水灌胃。RT-PCR法检测瘦素(Leptin)及Leptin受体(OB-Rb)mRNA的表达,Western blot法检测酪氨酸蛋白激酶2(JAK2)、信号转导和转录激活蛋白3(STAT3)的表达。结果与正常组比较,其余各组大鼠Leptin mRNA、OB-Rb mRNA、JAK2、STAT3表达明显增强(P<0.05);肝复健方低、中、高剂量组和秋水仙碱组较模型组大鼠上述各项指标明显降低(P<0.05),并且肝复健方高剂量组较肝复健方中、低剂量组和秋水仙碱组降低明显(P<0.05)。结论肝复健方抗HF的作用可能与其抑制Leptin、OB-Rb表达,进而抑制JAK2/STAT3信号通路有关。更多还原

【Abstract】 Objective To research the possible mechanism of Ganfujian Formula(formula for liver recovery)for liver fibrosis.Methods The SD rats were randomized into the normal group,model group,Ganfujian Formula low-dose group,Ganfujian Formula middle-dose group,Ganfujian Formula high-dose group and colchicines group,with 10in each.The liver fibrosis model was established with intraperitoneal injection of 0.5ml pig serum twice weekly for 8weeks in each group except the normal group.Both the normal and model groups were gavaged with 10ml/kg normal saline respectively.The Ganfujian Formula low-dose,middle-dose and high-dose groups were gavaged with 1.4g/ml,2.8g/ml and 4.2g/ml Ganfujian Formula decoction respectively.The colchicine group was given 0.154mg/kg suspension liquid of colchicine tablets.The expressions of leptin mRNA and leptin receptor(OB-Rb)mRNA were detected with reverse transcription-polymerase chain reaction.The expressions of Janus kinase 2(JAK-2)and signal transducer and activator of transcription 3(STAT3)were detected with Western blot.Results Comparing with the normal group,the expressions of leptin mRNA,OB-Rb mRNA,JAK2and STAT3were significantly increased in other groups(P<0.05).Comparing with the model group,the expressions of leptin mRNA,OB-Rb mRNA,JAK2and STAT3were significantly decreased in the Ganfujian Formula low-dose,middle-dose and high-dose groups and colchicine group(P<0.05),especially in the Ganfujian Formula high-dose group(P<0.05).Conclusion The possible mechanism of Ganfujian Formula for liver fibrosis is inhibiting the expressions of leptin and OB-Rb and thereby inhibiting JAK2/STAT3signaling pathway.更多还原