【摘要】 目的研究人参皂苷Rb1对大鼠局灶性脑缺血再灌注损伤(ischemia-reperfusion injury,I/R)后脑梗死体积及脑组织和血清中炎症因子IL-1β的影响。方法采用线栓法建立大鼠I/R模型,将SD大鼠随机分为假手术组,模型组,人参皂苷Rb1低(20 mg/kg)、中(40 mg/kg)、高剂量(80 mg/kg)组,每组12只。假手术组大鼠仅做大脑中动脉栓塞手术,但不插入线栓。除假手术组外,另4组均接受线栓法制备大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型,2 h后恢复大脑中动脉再灌注;人参皂苷Rb1各剂量组于脑缺血即刻腹腔注射,模型组给予等量生理盐水。观察各组大鼠脑缺血2 h再灌注损伤后24 h大鼠神经缺损程度评分、脑梗死体积、脑组织及血清IL-1β蛋白表达。结果与假手术组比较,模型组神经功能缺损程度评分提高(P<0.01),脑组织IL-1β阳性细胞数增加,血清IL-1β含量升高(P<0.05)。与模型组比较,人参皂苷Rb1中、高剂量组神经缺损程度评分降低(P<0.05,P<0.01),人参皂苷Rb1各剂量组梗死体积均缩小,脑组织IL-1β阳性细胞数减少,血清IL-1β含量降低(P<0.01,P<0.05)。与人参皂苷Rb1低剂量组比较,高剂量组神经功能缺损程度评分降低,梗死体积缩小,血清IL-1β含量升高(P<0.01,P<0.05)。结论人参皂苷Rb1(20、40、80 mg/kg)可能通过下调IL-1β的表达有效缓解大鼠局灶性脑I/R。更多还原

【Abstract】 Objective To investigate the effect of ginsenoside Rb1 on cerebral infarction volume as well as IL-1β in the brain tissue and sera of focal cerebral ischemia / reperfusion( I / R) injury model rats. Methods The I / R rat model was established by using thread according to Zea-Longa. SD rats were randomly divided into five groups,i. e.,the sham-operation group,the model group,the low dose ginsenoside Rb1( 20 mg / kg) group,the medium dose ginsenoside Rb1 group( 40 mg/kg),and the high dose ginsenoside Rb1 group( 80 mg/kg),12 in each group. Rats in the sham-operation group only received middle cerebral artery occlusion( MCAO) but without thread insertion. The MCAO model was prepared in the rest 4 groups,followed by MCAO 2 h later. Ginsenoside Rb1 at each dose was peritoneally administrated to rats in corresponding groups immediately after cerebral ischemia. Equal volume of normal saline was administered to rats in the sham-operation group. Rats’ cerebral infarction volume,integrals of neurologic defect degree,expression of IL-1β content in the brain tissue and sera were observed 24 h after 2-h cerebral I / R. Results In the model group,integrals of neurologic defect degree were improved(P < 0. 01),IL-1β positive cells in the brain tissue increased and serum IL-1β content elevated(P <0. 05),when compared with the sham-operation group. In comparison of the model group,integrals of neurologic defect degree were lowered in the medium dose and high dose ginsenoside Rb1 groups(P < 0. 05,P < 0. 01). The cerebral infarction volume was all shrunken in each ginsenoside Rb1 group,IL-1β positive cells in the brain tissue decreased,and IL-1β content in serum reduced(P < 0. 01,P < 0. 05). Compared with the low dose ginsenoside Rb1 group,integrals of neurologic defect degree decreased,the cerebral infarction volume shrunken,and IL-1β content in serum reduced in the high dose ginsenoside Rb1 group(P < 0. 01,P < 0. 05). Conclusion Ginsenoside Rb1( 20,40,80 mg / kg) might effectively release local cerebral ischemia by down-regulating the IL-1β expression.更多还原