【摘要】 目的研究松龄血脉康胶囊对自发性高血压大鼠(SHR)血压的影响以及对过氧化物酶体增殖物激活受体γ(peroxisome proliferator activated receptor-γ,PPARγ)的调节机制。方法 24只10周龄SHR大鼠随机分为空白组、中药组和西药组,每组8只。中药组灌胃松龄血脉康20mg/(kg·d);西药组灌胃雷米普利1mg/(kg·d);空白组给予同体积的生理盐水。每周测血压1次,4周后检测心脏超声;腹主动脉取血处死动物,荧光实时定量PCR技术检测肝脏PPARγ和血管紧张素Ⅱ1型受体(AT1R)mRNA的表达水平。免疫组织化学(SP法)染色观察PPARγ和AT1R蛋白的表达,蛋白印迹定量分析PPARγ和AT1R的含量。结果用药4周后,中药组血压降低,与空白组比较,差异有统计学意义(P<0.01)。中药降压效果不如西药明显,但整体来看,中药较西药降压稳定,能够使血压维持在相对稳定的水平;中药组心脏射血分数增高,并显著上调肝脏PPARγmRNA表达及肝脏PPARγ蛋白水平,与空白组比较,差异均有统计学意义(P<0.05,P<0.01);中药组AT1R mRNA表达明显抑制,AT1R蛋白水平下调,与空白组、西药组比较,差异均有统计学意义(P<0.01)。结论松龄血脉康胶囊通过上调PPARγmRNA的表达和蛋白合成,抑制AT1RmRNA的表达和蛋白合成,可能是其抑制血压升高,提高心脏射血分数的部分作用机制。更多还原

【Abstract】 Objective To study the effect of Songling Xuemaikang Capsule(SXC)on blood pressure of spontaneously hypertensive rats(SHR)and regulatory mechanisms for peroxisome proliferator activated receptor-γ(PPARγ).Methods Totally 24 10-week-old SHR rats were randomly divided into the blank control group,the Chinese medicine(CM)group,and the Western medicine(WM)group,8in each group.Rats in the CM group were administered with SXC at the daily dose of 20 mg/kg by gastrogavage.Those in the WM group were administered with ramipril at the daily dose of 1 mg/kg by gastrogavage.Those in the blank control group were administered with equal volume of normal saline.The blood pressure was measured once per week.The cardiac ultrasound was performed 4 weeks later.Rats were killed and then blood was sampled from abdominal aorta.mRNA expressions of liver PPARγ and angiotensinⅡtype 1receptor(AT1R)were detected by fluorescence real-time quantitative PCR.Protein expressions of PPARγand AT1Rwere detected using immunohistochemical assay(SP).The contents of PPARγand AT1R were quantitatively analyzed by Western blot.Results After 4weeks of treatment,the blood pressure decreased in the CM group,showing statistical difference when compared with the blank control group(P<0.01).CM was inferior to WM in lowering blood pressure.But as a whole,CM was more stable and could maintain blood pressure at a relatively stable level.The cardiac ejection fraction increased in the CM group,showing statistical difference when compared with the blank control group(P<0.05,P<0.01).The mRNA and protein expressions of liver PPARγwere upregulated in the CM group,showing statistical difference when compared with the blank control group(P<0.05,P< 0.01).CM could obviously inhibit the AT1RmRNA expression,and down-regulate the protein expression of AT1R,showing statistical difference when compared with the blank control group and the WM group respectively(P<0.01).Conclusion SXC decreased blood pressure and improved the cardiac ejection fraction,which might be partially achieved by up-regulating the PPARγmRNA expression and protein synthesis,and inhibiting the AT1RmRNA expression and AT1Rprotein synthesis.更多还原