【摘要】 目的评价通脑活络针刺疗法对大鼠急性脑梗死Bcl-2、Caspase-3的影响。方法将264只大鼠随机分为5组,分别为通脑活络针刺组(针刺组)72只、溶栓组72只、体针组72只、缺血对照组24只、假手术组24只,其中针刺组、溶栓组、体针组、缺血对照组均选用造模成功大鼠。针刺、溶栓、体针3组又分别按发病时间不同分为≤1.5h、1.5+～2h、2+～3h3个亚组。治疗后6、24、72h分别进行神经行为学评分,并在24、72h时点运用免疫组化法测脑组织Caspase-3、Bcl-2凋亡相关蛋白。结果在神经功能评分改善方面:与本组治疗前比较,发病<2h内,针刺组和溶栓组治疗后6h差异有统计学意义(P<0.05);发病2+～3h内,针刺组和溶栓组治疗后24、72h差异均有统计学意义(P<0.05);发病在3h内,针刺组在治疗后24、72h差异均有统计学意义(P<0.05,P<0.01)。在降低Caspase-3、提高Bcl-2蛋白表达方面:发病在2+～3h内,针刺组和溶栓组治疗72h后Bcl-2蛋白及Caspase-3蛋白差异均有统计学意义(P<0.05,P<0.01)。结论通脑活络针刺疗法在降低大鼠急性脑梗死神经行为学评分,降低Caspase-3、提高Bcl-2蛋白表达有较好疗效。更多还原

【Abstract】 Objective To evaluate effects of Tongnao Huoluo acupuncture therapy (THAT) on Bcl2 and Caspase-3 rats with acute cerebral infarction (ACI). Methods Totally 264 SD rats were randomly divided into 5 groups,i.e. the THAT group (n =72), the thrombolysis group (n =72), the body acupuncture group (n =72), the ischemia control group (n =24), and the sham-operation group (n =24). Successfully modeled rats were recruited in all groups except the sham-operation group. Rats in the THAT group, the thrombolysis group, and the body acupuncture group were divided into 3 subgroups according to the disease occurrence time, i.e., ≤1.5 h THAT group, 1.5+-2 h THAT group, and 2+-3 h THAT group. The neuroethological scores were assessed at 6, 24, and 72 h after treatment. The expressions of Bcl2 and Caspase-3 were detected using immunohistochemical staining at 24 and 72 h respectively. Results In aspect of improving scores of neurological functions: At 6 h after treatment within 2 h after the disease occurrence, the neuroethological scores were lowered more obviously in the thrombolysis group than in the THAT group (P <0.05). There was statistical difference at 24 and 72 h within 2-3 h after the disease occurrence between the THAT group and the thrombolysis group (P <0.05). Compared with before treatment, there was statistical difference at 24 and 72 h within 3 h after the disease occurrence (P <0.05, P <0.01). In aspect of lowering the expression of Caspase-3 and elevating the expression of Bcl2: There was statistical difference in lowering the expression of Caspase-3 and elevating the expression of Bcl2 between the THAT group and the thrombolysis group at 72 h within 2-3 after the disease occurrence (P <0.05,P <0.01). Conclusion THAT showed favorable effects in lowering neuroethological scores, lowering expression of Caspase-3, and elevating the expression of Bcl2 of ACI rats.更多还原