【摘要】 目的观察人参、川芎组合对体外培养氧糖剥夺/复氧大鼠神经干细胞细胞外信号调节激酶(ex-tracellular-signal-responsive kinase,ERK)信号通路和增殖、活力的影响。方法神经干细胞培养分为5组:正常对照组、氧糖剥夺/复氧组、氧糖剥夺/复氧加人参药物血清组、氧糖剥夺/复氧加川芎药物血清组、氧糖剥夺/复氧加人参川芎药物血清组。免疫印迹方法观察ERK及p-ERK蛋白表达水平,5-溴脱氧尿核苷(5-bro-modeoxyuridine,BrdU)掺入法观察神经干细胞的增殖,噻唑蓝(methyl thiazolyl tetrazolium,MTT)检测细胞活性。结果 ERK磷酸化水平于复氧后10min、30min一过性升高,但4h、6h、1d各时间点均降至正常水平;取复氧后6h时间点观察发现,相比氧糖剥夺/复氧组,人参、川芎、人参川芎含药血清均可升高ERK磷酸化水平,促进复氧后1d时神经干细胞的增殖,提高其活力,且人参川芎联合运用优于单用人参和单用川芎。结论益气活血药物人参川芎可能通过ERK信号通路促进氧糖剥夺、复氧后大鼠神经干细胞的增殖,提高细胞活力,效果优于单用益气药物人参和活血药物川芎。更多还原

【Abstract】 Objective To observe the effects of ginseng and Ligustrazine drug containing serum on the proliferation, vitality, and extracellular-signal-responsive kinase (ERK) pathway in neural stem cells undergoing in vitro oxygen-glucose deprivation/reoxygenation culture. Methods The cultured neural stem cells were randomly divided into 5 groups, i.e., the normal control group (Group A), the oxygen-glucose deprivation/reoxygenation group (Group B), the oxygen-glucose deprivation/reoxygenation+ginseng serum group (Group C), the oxygen-glucose deprivation/reoxygenation+Ligustrazine serum group (Group D), and oxygen-glucose deprivation/reoxygenation+ginseng and Ligustrazine drug serum group (Group E). The protein expression levels of ERK and phosphorylated ERK (p-ERK) were observed using immunoblotting. The proliferation of neural stem cells was observed using 5-bro-modeoxyuridine (BrdU) incorporation assay. The vitality of neural stem cells was detected using methyl thiazolyl tetrazolium (MTT) colorimetry. Results The p-ERK level increased transiently at 10 min and 30 min after reoxygenation, but it decreased to the normal level at 4 h, 6 h, and 1 day, respectively. Compared with Group B, the p-ERK level at 6 h after reoxygenation could be elevated in Group C, D, and E. The proliferation and the vitality of neural stem cells at 1 day after reoxygenation could be enhanced. Furthermore, the effects of combination of ginseng and Ligusticum were better than those of using ginseng or Ligusticum alone. Conclusions Combination of ginseng and Ligusticum could promote the proliferation and vitality of rats′ neural stem cells undergoing oxygen-glucose deprivation/reoxygenation culture through ERK signal pathway. Its effects was better than that of using ginseng or Ligusticum alone.更多还原