【摘要】 目的:研究脑舒胶囊对VD模型大鼠的中枢免疫炎症反应的抑制作用。方法:反复夹闭双侧颈总动脉加硝普钠降压复制VD大鼠模型;Morris水迷宫检测大鼠学习记忆力;放射性免疫法测定血清IL-6含量;ELISA法测定血清sIL-2R含量;免疫组织化学检测TNF-α阳性表达。结果:与假手术组比较,模型组大鼠血清IL-6及sIL-2R含量明显增加,与模型组相比,0.36g/kg、0.72g/kg、1.44g/kg脑舒胶囊均可降低大鼠血清IL-6及sIL-2R含量,具有一定剂量依赖性。光镜下(×400)下,模型组大鼠海马CA1区胞浆及胞膜棕褐色颗粒明显增加,各剂量脑舒胶囊治疗组大鼠海马CA1区胞浆及胞膜棕褐色颗粒明显减少;与假手术组比较,模型组TNF-α阳性反应的平均光密度(ID)均明显降低,各治疗组TNF-α阳性反应的ID均下降,且有一定剂量依赖性。结论:脑舒胶囊对VD大鼠中枢免疫炎症反应有较好抑制作用。更多还原

【Abstract】 Objective:To research the inhibition of Naoshu Capsule on central immune inflamation in rats with VD.Method: VD rat model was induced by closure both common carotid arterys and reperfusion several times,acuity of learning and memory of rats was measured with Morris water maz;serumal IL-6 was detected by Radio Immunoassay and serumal sIL-2R were detected by ELISA,expression of TNF-α was observed by immunohistochemistry.Results: Compared with sham operation group,serumal IL-6 and sIL-2R increased in model group(P<0.01).Compared with model group,Naoshu Capsule(0.36,0.72,1.44g/kg) decreased serumal IL-6 and sIL-2R(P<0.05,P<0.01).In light microscope(×400),in model group,brown granules in endochylema and membrane of VD rats’ hippocampus CA1 domain increased,and that decreased obviously in Naoshu Capsule groups.Compared with sham operation group,the ID of TNF-α of model group decreased obviously(P<0.01),and the ID of TNF-α of Naoshu Capsule groups decreased.Dosage dependence was observed in Naoshu Capsule group.Conclusion: Naoshu Capsule can inhibite central immune inflamation of VD rats.更多还原