【摘要】 目的探讨UrocortinⅠ预处理对缺氧/复氧后成年大鼠心肌细胞线粒体膜电位的影响。方法通过离体心脏灌注装置分离成年大鼠心肌细胞,培养24 h后随机分成4组:正常组(Nor组)在37℃培养箱中持续培养155min;余3组均给予缺氧40 min、复氧60min制作缺氧/复氧模型;I/R组不做任何处理;UrocortinⅠ组(UcnⅠ)和5-羟葵酸组(5-HD+UcnⅠ组)在缺氧前均给予UcnⅠ处理30 min,其中5-HD组先给予5-HD处理5 min后行30 min的UcnⅠ处理。各组在复氧末时加入JC-1荧光探针,用激光共聚焦显微镜检测心肌细胞线粒体膜电位。结果 Nor组心肌细胞线粒体膜电位较高(P<0.01);与Nor组相比I/R组线粒体膜电位显著下降(P<0.01);而UcnⅠ组线粒体膜电位明显高于I/R组和5-HD+UcnⅠ组(P<0.01)。结论 UcnⅠ预处理可以稳定成年大鼠缺氧/复氧后心肌细胞线粒体膜电位,其机制可能与开放线粒体敏感性钾通道有关。更多还原

【Abstract】 Objective Explore the influence of the urocortin I(Ucn I)pretreatment on mitochondrial membrane potential in myocardial cells from adult male SD rats after hypoxia / reoxygenation.Methods Adult rat cardiomyocytes were isolated by the Langendorff isolated heart perfusion apparatus,and cultured for 24 hours.The cells were randomized into 4 groups: In Nor group,the cultured cells were constantly cultured at temperature of 37 ℃ for 155 minutes.The other three grous were given 40 min oxygen deprivation and subsequently reoxygenation for 60 min to produce hypoxia / reoxygenation model.There was no any extra treatments on I / R group.Ucn I group and 5-HD group were given Ucn I preconditioning for 30 minutes before hypoxia.In 5-HD group,the cultured cells was reacted with 5HD for 5 min before UcnⅠ.At the end of reoxygenation process,the cultured cardiomyocytes were mixed with JC-1 and the mitochondrial membrane potential of cultured cardiomyocytes were detected via the confocal microscope.Results The inflorescences intensity of mitochondrial membrane potential were significantly higher in Nor group than that in I / R group,UcnⅠgroup and 5-HD group(P <0.01).However,the florescences intensity of mitochondrial membrane potential in UcnⅠgroup was much higher than in I / R Group and 5-HD group(P < 0.01).Conclusion UcnⅠpreconditioning can stable the mitochondrial membrane potential of adult rat myocardial cells after hypoxia / reoxygenation,afford myocardial protection,which may relate to the opening of ATP sensitive potassium channel.更多还原