【摘要】 目的筛查严重少精子症和无精子症患者Y染色体AZF区域微缺失的发生情况,探讨Y染色体微缺失患者生殖激素的水平。方法对195例严重少精子症和80例无精子症患者进行Y染色体无精子因子(azoospermia factor,AZF)微缺失分析,同时用化学发光法测定生殖激素水平。结果 275例患者中发生AZF微缺失患者21例,检出率为7.6%,其中少严重精子症15例,无精子症6例。21例AZF微缺失情况:AZFa区缺失3例;AZFb+c+d区缺失4例;AZFc+d区缺失11例;AZFd区缺失3例。Y染色体AZFb+c+d区缺失患者的卵泡刺激素(FSH)值(46.2±10.3)mIU/mL显著高于无Y染色体缺失患者(17.6±15.2)mIU/mL和AZFa区、AZFc+d区、AZFd区缺失患者(15.8±5.7)mIU/mL,差异具有统计学意义(P<O.05)。结论在无精与严重少精症患者中Y染色体的微缺失以AZFc区和AZFd区缺失最为常见,Y染色体AZFb+c+d区缺失是引起高卵泡刺激素的重要原因之一。更多还原

【Abstract】 Objective: To screen for Y chromosome microdeletions in patients with severe oligospermia or azoospermia,and explore the levels of gonadal hormone about patients of Ychromosome microdeletion. Methods: Y chromosome microdeletions among 195 patients with severe oligospermia and 80 patients with azoospermia were analyzed,and the levels of gonadal hormone were detected by chemilu- minescent met- hod. Results: The rate of microdeletion was 7. 6%( 21 out of 275 patients),of which 15 were severe oligospermia,6 were azoospermia. The deletion was on AZFa in 3 patient,AZFb + c + d in 4patients,AZFc + d in 11 patients,AZFd in 3 patient,respectively. The level of FSH( 46. 2 ± 10. 3mIU / mL) in the AZFb + c + d deletion group was significantly higher than that in the group without Y chromosome deletion( 17. 6 ± 15. 2mIU / mL) and the other types of deletion( 15. 8 ± 5. 7 mIU / mL)( P < 0. 05). Conclusion: The common microdeletion regions were AZFc and AZFd on Y chromosome in azoospermia or severe oligozoospermia. The microdeletion of AZFb + c + d was one of the important causes of the high leve1 of FSH.更多还原