【摘要】 目的探讨MIF、MMP-3及IL-8联合检测在胎膜早破的意义及与绒毛膜羊膜炎之间的关系。方法采用免疫组化法检测75例PROM分娩组和70例非PROM分娩组胎膜中MIF,MMP-3及IL-8的分布和表达,采用HE染色的方法确诊绒毛膜羊膜炎。结果 PROM组胎膜MIF、MMP-3、IL-8表达均明显高于对照组,经统计学分析,两组间有显著性差异(P<0.001)。PROM绒毛膜羊膜炎组胎膜MIF、MMP-3、IL-8表达均明显高于PROM非绒毛膜羊膜炎组,经统计学分析,两组间有显著性差异(P<0.05)。结论 MIF、MMP-3、IL-8在胎膜早破的发病机制中起着重要的作用,MIF、MMP-3、IL-8与绒毛膜羊膜炎有密切关系。更多还原

【Abstract】 Objective: To explore the significance of combine detection of MIF,MMP-3 and IL-8 in premature rupture of membranes and chorioamnionitis,and their relationship with premature rupture of fetal membranes(PROM) and chorioamnionitis.Methods: Using immunohistological method to investigate the distributions and expressions of MIF,MMP-3,IL-8 in the specimens of 75 PROM patients and 70 non-PROM patients.Using HE staining to define histopathology diagnosis: chorioamnionitis and unchorioamnionitis.Results: The quantity of MIF,MMP-3,IL-8 positive cells in fetal membrane in PROM patients were significantly higher than those in non-PROM patients(P<0.001).The quantity of MIF,MMP-3,IL-8 positive cells in fetal membrane in PROM patients with chorioamnionitis were obviously higher than those in PROM patients without chorioamnionitis(P<0.05).Conclusion: MIF,MMP-3,IL-8 all play an important role in pathogenesis of PROM,they are related to chorioamnionitis closely.更多还原