【摘要】 目的:探讨三氧化二砷(ATO)、全反式维甲酸(ATRA)及粒细胞集落刺激因子(G-CSF)(A/G)联合使用,诱导NB4细胞末端分化的分子机理。方法:台盼蓝染色记录细胞生长,吉姆萨染色观察细胞的形态学变化,流式细胞仪分析确定细胞分化分子标志及凋亡,Western blot观察RAR α蛋白的表达。结果:NB4细胞经过ATO+A/G处理,出现形态学末端分化特征和免疫表型的成熟。Western blot结果显示,RAR α基因是ATO特定的靶向目标。结论:ATO联合ATRA/G-CSF能够诱导NB4细胞末端分化,其机理与调节RAR α的表达有关。更多还原

【Abstract】 Objective:To investigate the molecular mechanism of ATO in combination with ATRA /G-CSF(A /G) induced terminal differentiation of NB4 cells.Methods:Trypan blue staining was used to record cells growth,Cell morphological features were observed by Giemsa staining,molecular marker of cell differentiation and cell apoptosis were determined by flow cytometry(FCM),the expression of RAR α was detected by Western blot.Results:NB4 cells present the morphological terminal differentiation and the immune phenotype maturation treated by ATO + A / G.Western blot results showed that RAR α gene was the specific goal.Conclusion:ATO combined with A / G could induce terminal differentiation of NB4 cells,and its mechanism is related to the regulation of RAR α expression.更多还原