【摘要】 目的:观察中药益坤宁方对围绝经期大鼠卵巢细胞色素P450芳香化酶(Aromatase cytochrome P450,P450arom)的调节作用。方法:建立自然衰老的大鼠模型,将模型分为青年对照组、模型组、益坤宁组和西药对照组4组,益坤宁组和西药对照组分别给予中药益坤宁汤剂和西药利维爱混悬液治疗。4周后,采用免疫组织化学及Western Blot方法检测大鼠卵巢P450arom蛋白表达情况;采用放射免疫法测定大鼠空腹血清雌二醇(E2)水平。结果:P450arom蛋白在益坤宁组大鼠卵巢上均多呈阳性表达,与模型组比较均具有显著性差异(P<0.01);与西药对照组比较差异不具有统计学意义(P>0.05)。益坤宁组大鼠空腹血清雌二醇(E2)水平升高,与模型组比较差异显著(P<0.01),与西药对照组比较差异不具统计学意义(P>0.05),但其E2水平仍然低于青年对照组,差异具有显著性(P<0.05)。结论:中药益坤宁可促进围绝经期大鼠卵巢P450arom蛋白的表达。中药益坤宁可提高大鼠血清雌二醇(E2)的水平。中药益坤宁可通过促进卵巢P450arom的表达而利于雌激素的生成。更多还原

【Abstract】 Objective:To observe the effect of traditional Chinese medicine Yikunning on ovarian cytochrome P450 aromatase(P450arom) during perimenopausal period.Methods:Select natural aging rat models.Then randomly divide them into model group,Yikunning group,the control group of western medicine and the control group of youth.Yikunning and liviai were given to Yikunning medicine group and the control group of western medicine.Four weeks later,we detected the expression of P450arom mRNA and protein on the ovarians with the methods of iimmunohistochemistry and western blot.At the same time,we detected the estradiol(E2) level with RIA determination.Results:P450arom and protein mostly expressed positively on ovarians in Yikunning group compared with the model group,and there was a significant difference(P < 0.01);Compared with the control group of western medicine,the difference did not have a statistical significance(P > 0.05).Yikunning rats’ serum estradiol(E2) levels increased,compared with the model group,the difference was significant(P < 0.01),compared with the control group of western medicine there was no significant difference(P > 0.05),but its E2 levels were still below the young-aged control group,and the difference was significant(P < 0.05).Conclusion:Chinese medicine Yikunning could promote the expression of ovarian P450arom during perimenopausal period.Yikunning could raise serum estradiol(E2) level.Yikunning could promote the production of estrogen through promoting the expression of ovarian P450arom.更多还原