【摘要】 目的采用柱前衍生高效液相色谱法测定克拉霉素血药浓度。方法克拉霉素血浆样品以正己烷-正丁醇混合溶液萃取后,与9芴甲氧羰酰氯（FMOC-CL）衍生化后进样。色谱柱为Hypersil^TMODS2柱（4.6mm×250mm,5μn）,柱温:62℃;流动相:0.05mol/LNH₄H₂PO₄缓冲液（PH6.72）-乙腈=38:62（v/v）,流速2.5 mL/min:激发波长:265m,发射波长:315nm。结果克拉霉素血药浓度在50³000ng/mL范围内,浓度与峰面积比有良好的线性关系,最低检测浓度为30ng/mL,平均回收率为97.9¹00.2%,日内RSD≤4.1%,日间RSD≤6.8%。结论本方法简单、快速、准确,可用于克拉霉素临床药动学研究。更多还原

【Abstract】 Objective To establish a HPLC method with pre-column derivatization to measure the plasma concentration of clarithromycin.Methods A n-hexane and n-butanol extract of plasma was treated with FMOC-CL and chromatographed on a HypersilTM ODS2 column(4.6mm×250mm,5μm) using an eluent composed of acetonitrile- 0.05mol/L phosphate buffer(PH6.72)(62:38).The column temperature was at 62°C and flow rate of eluent was 2.5mL/min.The exciation and emission wavelength were 265nm and 315nm,respectively.Results The assay procedure was shown to produce linear calibration curves over the range 50ng/mL to 3000ng/mL of clarithromycin in plasma.The limit of detection was 30ng/mL The recovery rate was 97.9~100.2%.The coefficients of variation within a day and between days did not exceed 4.1%,6.8%,respectively.Conclusion This method is found to be easy,convenient and precise for clinical pharmacokinetics study of clarithromycin.更多还原