【摘要】 目的:制定骨康颗粒质量标准。方法:采用薄层色谱法鉴别补骨脂、续断、牛膝,高效液相色谱法测定淫羊藿苷、补骨脂素、异补骨脂素的含量。色谱柱:大连依利特Hypersil ODS2（150mm×4.6mm,5μm）,流动相:甲醇-乙腈-水（5:25:70）、乙腈.水（30:70）;流速:1ml·min^-1;检测波长:270nm、246nm;柱温:35℃。结果:TLC色谱能明显检出补骨脂、牛膝、续断。淫羊藿苷、补骨脂素、异补骨脂素分别在0.112⁰.336μg（r=0.9996）、0.073⁰.220μg（r=0.9998）、0.076⁰.229（r=0.9997）范围内呈现良好线性关系,淫羊藿苷的平均回收率为98.87%（RSD=1.73%）;补骨脂素、异补骨脂素的平均回收率分别为100.57%（RSD=1.47%）和100.38%（RSD=1.26%）。结论:本方法简便,准确,重现性好,可用于本品的质量控制。更多还原

【Abstract】 Objective:To establish the quality standard for Gukang granules.Method:TLC was used for the identification of fructus psoraleae,radix dipsaci and achyranthes.An HPLC method was applied in the content determination of icariin。psoralen and isopsoralen.The chromatographic column was a Dalian Elite Hypersil ODS2（150mm×4.6 mm,5μm）,the mobile phase was methanolacetonitrile.water（5:25:70）for icariin,and acetonitrile.water（30:70）for psoralen and isopsoralen.The flow rate Was 1.0ml·min^-1,the detection wavelenZth was 270nm and 246nm,respectively and the column temperatUre was 35℃.Result:The TLC could detect fructus psoraleae,radix dipsaci and achyranthes.Icariin,psoralen and isopsoralen showed good linear relationship within the range of0.112-0.336μg（r=0.999 6）,0.073-0.220μg（r=0.999 8）and0.076-0.229μg（r=0.9997）,respectively.The average recovery was 98.87%（RSD=1.73%）,100.57%（RSD=1.47%）and 100.38%（RSD=1.26%）,respectively.Conclusion:The method is simple,accurate and repeatable,and can be used in the quality control of Gukang grandes.更多还原