【摘要】 目的:研究黄芪对急性肺损伤大鼠肺组织中角质细胞生长(Keratinocyte GrowthFactor,KGF)表达的影响,并探讨其可能的机制。方法:SPF级Wistar大鼠60只,随机分为正常对照组(Normal Saline,NS组)、模型组(Acute Lung Injury,ALI组)、黄芪高剂量组(large does of astragalus membranaceous)、黄芪中剂量组(middle does of astragalus membranaceous)、黄芪低剂量组(small does of astragalus membranaceous)、地塞米松组(Dexamethasone,DEX组)。通过静脉内注射油酸(Oleic Acid,OA)0.1mlkg-1建立大鼠模型,利用光镜观察肺部组织形态学变化,计算肺系数(Lung Index,LI),酶联免疫吸附法(ELISA)测定血清中KGF含量,采用实时荧光定量PCR法检测肺组织KGFmRNA表达情况。结果:黄芪高、中剂量能提高血清KGF含量(P<0.01),降低肺系数(P<0.01),增强肺组织KGFmRNA表达(P<0.05),减轻肺组织病理损伤。黄芪低剂量的效果不如高、中剂量(P<0.05)。结论:黄芪能够明显减轻油酸所致急性肺损伤的炎症反应,其药理作用机制与上调肺内KGF的表达,从而刺激肺泡Ⅱ上皮细胞(ⅡAlveolar EpithelialType Cells,AECII)增生有关。更多还原

【Abstract】 Objective: To investigate the effect and mechanism of Huangqi on acute lung injury (ALI) rat induced by OA. Methods: 60 SPF Wistar rats were randomly divided into 6 groups, control group, model group, LA group, MA group, SA group and dexamethasone group. A rat model of ALI was established by intravenous injection of oleic acid with a dose of 0.1ml·kg-1 . Light microscope was used to examine histological changes and lung index was calculated by weighting method. KGF levels in serum were determined using commercial enzyme-linked immunosorbent assay kits. The expression of KGF genes were detected by real-time PCR. Results: LA group and MA group improved the production of KGF in whole blood (P<0.01), reduced the lung index (P<0.01) and intensified KGFm RNA expression (P<0.05) as compare with the ALI group. Pathological changes of ALI were reduced significantly in AM intervention groups. SM group was lower than the effect of LA group and MA group (P<0.05). Conclusion: Huangqi could alleviate oleic acid induced acute lung injury in rats,and its pharmacological action mechanism may be related to raise the lungs KGF expression,so as to stimulate Ⅱ alveolar epithelial type cells (AECⅡ) proliferation.更多还原