【摘要】 目的探讨apoE-/-小鼠颈总动脉斑块处趋化因子Fractalkine(FKN)和分子标志物CD11c的表达与动脉粥样硬化(AS)严重程度的关系。方法高脂饲养apoE-/-小鼠12周,建立动物模型,同时以普食饲养的apoE-/-小鼠作为对照组。实验结束后,检测小鼠血脂、颈总动脉斑块面积和血管狭窄程度,评价实验动物AS严重程度。然后,应用免疫组织化学的方法检测斑块处FKN、CD11c的表达情况。结果与对照组相比,实验组AS斑块面积和血管狭窄率均升高(约4倍和2倍);实验组FKN表达升高,是对照组的2倍多;实验组斑块内CD11c阳性细胞数是对照组的近4倍,两组比较差异均具有统计学意义(P<0.05)。结论在AS斑块发生、发展过程中,趋化因子FKN表达升高,并且斑块处的树突状细胞增多,可能在AS的发病过程中发挥重要作用。更多还原

【Abstract】 Objective To explore the association of fractalkine(FKN) and CD11c expressions oncommon carotid artery atherosclerotic plaques from apoE-/-mice with the severity of atherosclerotic lesions.Methods Totally 24 apoE-/-mice were divided into two groups and fed on a high-fat diet or a normal diet for 12 weeks.Then the blood lipids as well as the plaque area and vascular stenosis rate of the common carotid artery were measured to evaluate the severity of atherosclerotic lesions of the animals.Moreover,immunohistochemical staining was performed to examine the levels of FKN and CD11c expression.Results The plaque areas and vascular stenosis rates of the common carotid artery in the experimental group were remarkably larger than those in control group(about 4-fold and 2-fold,respectively).The level of FKN expression in the experimental group was 2 times of that in the control group(P<0.05),and the number of CD11c+ cells in the plaques in the experimental group was about 4 times of than in the control group(P<0.05).Conclusion The expressions of chemokine and FKN remarkably increase in apoE-/-atherosclerotic plaques,suggesting that chemokine and FKN may paly important roles in the development of atherosclerosis.更多还原