【摘要】 目的探讨沥青烟对小鼠血清及脑组织中谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)活力及过氧化氢(H2O2)、丙二醛(MDA)含量的影响。方法将40只成年雄性小鼠按体重随机分为4组,分别为对照组(0 mg/m3)和低剂量(41 mg/m3,1/4 LD0)、中剂量(82 mg/m3,1/2 LD0)及高剂量(165 mg/m3,LD0)染毒组,每组10只。采用1 m3静式染毒柜行呼吸道吸入染毒,2 h/d,连续8周。测定各组小鼠血清和脑组织中GSH、H2O2、MDA含量及GSH-Px活力。结果与对照组比较,染毒组小鼠血清和脑组织匀浆中GSH含量和GSH-Px活力显著下降,H2O2、MDA含量显著升高。结论沥青烟可致小鼠脑组织细胞发生氧化性损伤,使机体的抗氧化能力降低。更多还原

【Abstract】 [Objective]To investigate the effect of bitumen fume on activities of glutathione(GSH),glutathione peroxidase(GSH-Px) and contents of hydrogen peroxide(H 2 O 2) and maleic Dialdehyde(MDA).[Methods]A total o f 40 adult male mice were randomly divided into 4 groups,including control group(0 mg / m3),low dose group(41 mg/m3,1/4 LD 0),medium dose group(82 mg / m3,1/2 LD 0),high dose group(165 mg/m3,LD 0),with 10 in each group.The animals were exposed to bitumen fume by 1 m3motionless exposure cabinet through respiratory passage 2 h / d,for 8 weeks.At the end of 8th week,activities of GSH-Px,and contents of GSH,H 2 O 2,MDA of blood and brains tissue were examined.[Results]Compared with the control group,the GSH content and GSH-Px activity in blood and brains tissue of mice with exposure were decreased significantly,with the contents of H 2 O 2 and MDA were increased significantly.[Conclusion]Bitumen fume can cause oxidative damage on brain tissues of mouse,and can reduce its antioxidant ability.更多还原