【摘要】 目的了解清远市人类免疫缺陷病毒(HIV)抗体初筛实验与确证实验结果符合率,为提高实验室HIV抗体检测技术提供依据。方法对清远市2010—2011年经酶联免疫吸附试验(ELISA)初筛的273份标本HIV抗体阳性及可疑阳性的结果与确证实验的结果进行比较分析。结果 273份标本,经蛋白印迹(WB)试验确证HIV-1阳性257例,占94.14%;不确定9例,占3.3%;阴性7例,占2.56%。筛查阳性与确证阳性总体符合率为94.14%,确证条带gp160、gp120、p24出现率均有95%以上;出现8条以上条带的有166份,出现率为64.59%。结论初筛试剂检测HIV抗体与WB确证试验的检测结果符合率较高,但判断HIV抗体阳性或艾滋病必须经WB试验确证,以排除假阳性。更多还原

【Abstract】 [Objective]To understand the coincidence rate of HIV antibody screening and confirmed test in Qingyuan City,provide a basis for improving the HIV antibody examination technology.[Methods]The ELISA results of preliminary screening(273 HIV antibody positive samples and probable positive samples) in Qingyuan City from 2010-2011 were compared with the results of confirmed test.[Results]In 273 HIV antibody positive samples of screening,257 cases were confirmed by Western blot(WB) test,which accounted for 94.14%,while 9(3.3%) cases were uncertain and 7(2.56%) cases were negative.The total coincidence rate of screening and confirmed test for positive cases was 94.14%.The occurrence rate of gp160,gp120 and p24 reactive bands was over 95%.166 cases had more than 8 bands in WB test(64.59%).[Conclusion]The coincidence rate of HIV antibody screening and confirmed WB test is high.However,HIV antibody positive cases and AIDS cases must be confirmed by WB test to exclude false positive reaction.更多还原