【摘要】 目的:探讨黄芪总黄酮(TFA)对人肝癌HepG-2细胞生长抑制及凋亡诱导作用及其机制。方法:以不同浓度TFA处理HepG-2细胞,MTT法测定细胞增殖抑制率;流式细胞仪(FCM)检测细胞凋亡率;Westernblot检测凋亡相关蛋白Survivin、Bc1-2、Bax的表达。结果:TFA能够抑制体外培养的人肝癌细胞HepG-2生长、诱导细胞凋亡,其作用具有量效关系和时效关系。Western blot检测显示,随着TFA浓度的增加,细胞凋亡抑制蛋白Survivin和Bcl-2的表达量明显降低,而细胞凋亡促进蛋白Bax的表达量显著增加。结论:TFA对HepG-2细胞具有抑制增殖和诱导凋亡的作用,其机制与下调细胞凋亡抑制蛋白Survivin与Bcl-2的表达和上调细胞凋亡促进蛋白Bax的表达有关。更多还原

【Abstract】 Objective:To investigate the possible molecular mechanism in TFA inducing apoptosis of human hepatoma carcinoma cell line HepG-2.Methods:HepG-2 cells were treated by different concentrations of TFA,the inhibitory rate of cell proliferation was measured by MTT.The apoptosis rate was detected by FCM.The expressions of Survivin,Bcl-2 and Bax protein were detected by Western blot.Results:TFA can inhibit the proliferation and induce apoptosis of human hepatocellular carcinoma HepG-2 cells,which had a significant effect of time and concentration dependent manner.The expressions of Survivin and Bcl-2 were decreased,but the expression of Bax was increased.Conclusions:TFA can inhibit the proliferation and induce apoptosis of HepG-2 cells.The effects may be related to the down-regulation of survivin and Bcl-2 expressions and up-regulation of Bax expression.更多还原