【摘要】 目的:建立木瓜饮片HPLC指纹图谱,并比较不同炮制品指纹图谱的差异。方法:采用高效液相色谱法,流动相以乙腈-甲醇(7∶3)为有机相,0.03%磷酸水溶液为水相梯度洗脱,流速为0.8 mL/min,检测波长为300nm,柱温30℃。以"中药色谱指纹图谱相似度评价软件"建立了木瓜饮片的标准指纹图谱,并计算相似度。结果:方法的精密度、重复性与稳定性良好。木瓜饮片被聚为3类,各样品相似度均大于0.90。木瓜炮制后共有峰的数目变化不明显,而峰面积变化较明显。结论:该法稳定准确,为木瓜饮片质量标准的制订提供了科学依据。炮制温度和辅料对成分的溶出均有一定影响。更多还原

【Abstract】 Objective: To establish HPLC fingerprint of Chaenomelis Fructus and compare HPLC fingerprints of different processed products. Methods: The mobile phase consisted of acetonitrile-methanol( 7∶ 3)( A) and 0. 03% phosphoric acid( B) in a gradient mode. Flow rate was 0. 8 mL / min. The wavelength was set at 300 nm and column temperature was 30 ℃. The HPLC chromatograms were analyzed by the"traditional Chinese medicine fingerprint similarity calculation software". Results: The results of methodological study met technical requirements of HPLC fingerprint. Ten batches of samples were divided into three groups by hierarchical cluster analysis and the similarities exceeded 0. 90. The HPLC fingerprints of different processed products had obvious differences in area of common peaks but less differences in number of common peaks. Conclusion: The HPLC fingerprint method is stable,accurate and reliable and can be used for scientific quality control of Chaenomelis Fructus. The processing temperature and processing materials have effect on the dissolution of chemical compositions in Chaenomelis Fructus.更多还原