【摘要】 目的:观察中药方剂1号的免疫调节及抗氧化作用。方法:1、SPF级BALB/c小鼠随机分为对照组(蒸馏水)、阳性对照组(破壁灵芝孢子粉,1.0 g/kg)及中药方剂1号低(0.35 g/kg)、中(0.70 g/kg)、高(1.4 g/kg)剂量组,灌胃给药1次/d,持续30 d;通过脾脏和胸腺指数、脾淋巴细胞增殖、腹腔巨噬细胞吞噬鸡红细胞、碳廓清等指标考察中药方剂1号的免疫调节作用。2、SPF级BALB/c小鼠随机分为正常对照组、模型组(D-半乳糖致过氧化损伤)、破壁灵芝孢子粉阳性对照组及中药方剂1号低、中、高剂量组,给药1次/d,持续30 d;检测脑组织SOD活性、MDA及LPO含量,western blotting分析脑组织胞核Nrf2蛋白表达。结果:与对照组比较,中药方剂1号高剂量组和破壁灵芝孢子粉组动物胸腺及脾脏指数显著升高,各给药组淋巴细胞增殖和巨噬细胞吞噬功能均显著提高;中药方剂1号中、高剂量和阳性对照药能显著提高模型小鼠脑组织SOD活性、降低MDA和LPO含量、增加Nrf2蛋白表达,中药方剂1号低剂量能显著减少小鼠脑组织MDA含量,增加Nrf2蛋白含量。结论:中药方剂1号对免疫功能有良好的调节作用,同时可能通过调节脑组织Nrf2蛋白表达及其相关信号通路发挥抗氧化作用。更多还原

【Abstract】 Objective: To observe the effect of immunoregulation and anti-oxidation of Zhongyaofangji NO1( ZYFJ). Methods: 1.SPF BALB / C mice were randomly divided into control group( distilled water),positive control group( Broken Spore),ZYFJ low dose group( 0. 35 g / kg),middle dose group( 0. 70 g / kg) and high dose group( 1. 40 g / kg),with intragastric administration 1 time / d for30d; The spleen and thymus index,ability of spleen lymphocyte proliferation,phagocytosing chicken red blood cell( CRBC) of abdominal macrophage cell,carbon clearance were investigated. 2. SPF BALB / C mice were divided into normal control group,model control group( D-galactose induced peroxidation damage),model plus ZYFJ low dose,middle dose and high dose group,model plus positive control group( Broken Spore),with drug administration 1 time / d for 30 d. SOD activity,MDA and LPO content in brain tissue were tested while Nrf2 [Nuclear factor( erythroi D-derived 2)-like 2]protein expression in brain tissue nucleus was tested by western blotting. Results:The thymus index and spleen index in groups of ZYFJ high dose and positive control were higher than those in control group,the ability of lymphocyte proliferation and phagocytosis of macrophages were increased in all the other groups significantly compared with control group; The activity of SOD and Nrf2 protein expression level in brain tissue of model mice was increased,MDA and LPO contents were reduced in ZYFJ middle and high dose as well as positive control significantly,while the MDA content was reduced and Nrf2 protein was increased in low dose group. Conclusion: Appropriate dose of ZYFJ1 has good effect of immunoregulation,and plays a role of anti-oxidation probably by regulating Nrf2 protein expression in brain tissue and related signaling pathway.更多还原