【摘要】 目的:考察黄芩茎叶黄酮提取物(SSF)对过氧化氢(H2O2)诱导的大鼠原代皮层细胞损害的保护作用。方法:100μmol.L-1H2O2作用大鼠原代皮层细胞150 min,测定细胞存活率、形态、乳酸脱氢酶(lac-tate dehydrogenase,LDH)释放量、丙二醛(malondialdehyde,MDA)的产生、超氧化物歧化酶(superoxide dis-mutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)/Na+-K+-ATP酶的活性以及SSF对H2O2引起大鼠原代皮层细胞上述指标异常改变作用。结果:H2O2明显降低大鼠原代皮层细胞存活率,使细胞形态发生异常变化,增加LDH和MDA生成,显著降低SOD/GSH-Px/Na+-K+-ATP酶的活性。18～76μg.mL-1SSF预处理细胞24 h明显反转上述变化。结论:SSF对H2O2引起的神经损伤具有保护作用,该作用有利于神经退行性疾病的治疗。更多还原

【Abstract】 Objective: To investigate the effect of flavonoid extract from stem and leaf of Scutellaria baicalensis Georgi on H2O2-induced damage in primary rat cortical neurons.Methods: Rat cortical neurons were treated with 100 μmol·L-1 H2O2 for 150 min.Cell damage was evaluated by determining viability,morphology,lactate dehydrogenase(LDH) release,malondialdehyde(MDA) production,and the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) and Na+-K+-ATPase.Effects of the flavonoids extract on H2O2-induced damage in the neurons were assessed.Results: After exposure of the neurons to H2O2,cell viability reduced,abnormal changes in neuron morphology were found,LDH release and MDA production increased,and SOD,GSH-Px and Na+-K+-ATPase activities were decreased.Flavonoid extract(18~76 μg·mL-1 for 24 h) attenuated H2O2-induced damage,as the cell survival,and SOD,GSH-Px and Na+-K+-ATPase activities were raised,and MDA production and LDH release were lowered.Conclusion: Flavonoid extract from stem and leaf of Scutellaria baicalensis Georgi has a protective effect on H2O2-induced neuronal damage,further supporting its therapeutic potentials for neurodegenerative diseases.更多还原